BMRB Entry 50819

Name: N-terminal domain of p50 subunit of NF-kappaB
Published: 2021-06-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50819

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

N-terminal domain of p50 subunit of NF-kappaB

Deposition date:

2021-03-10

Original release date:

2021-06-25

Authors:

Dhaka, Nitin; Joseph, David; Kakita, Veera Mohana; Mukherjee, Sulakshana

Citation:

Citation: Raza, Tahseen; Dhaka, Nitin; Joseph, David; Dadhwal, Prikshat; Kakita, Veera Mohana Rao; Atreya, Hanudatta; Mukherjee, Sulakshana. "Insights into the NF-kB-DNA Interaction through NMR Spectroscopy." ACS Omega 6, 12877-12886 (2021).
PubMed: 34056439

Assembly members:

Assembly members:
entity_1, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPYLQILEQPKQRGFRFRYV CEGPSHGGLPGASSEKNKKS YPQVKICNYVGPAKVIVQLV TNGKNIHLHAHSLVGKHCED GVCTVTAGPKDMVVGFANLG ILHVTKKKVFETLEARMTEA CIRGYNPGLLVHSDLAYLQA EGGGDRQLTDREKEIIRQAA VQQTKEMDLSVVRLMFTAFL PDSTGSFTRRLEPVVSDAIY DSKAPNA

Data sets:

heteronucl_NOEs
- heteronucl_NOEs_1
heteronucl_T1_relaxation
- heteronucl_T1_relaxation_1
heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1

Data type	Count
T1 relaxation values	101
T2 relaxation values	101
heteronuclear NOE values	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p50NTD	1

Entities:

Entity 1, p50NTD 207 residues - Formula weight is not available

1

GLY

PRO

TYR

LEU

GLN

ILE

LEU

GLU

GLN

PRO

2

LYS

GLN

ARG

GLY

PHE

ARG

PHE

ARG

TYR

VAL

3

CYS

GLU

GLY

PRO

SER

HIS

GLY

LEU

PRO

4

GLY

ALA

SER

GLU

LYS

ASN

LYS

SER

5

TYR

PRO

GLN

VAL

LYS

ILE

CYS

ASN

TYR

VAL

6

GLY

PRO

ALA

LYS

VAL

ILE

VAL

GLN

LEU

VAL

7

THR

ASN

GLY

LYS

ASN

ILE

HIS

LEU

HIS

ALA

8

HIS

SER

LEU

VAL

GLY

LYS

HIS

CYS

GLU

ASP

9

GLY

VAL

CYS

THR

VAL

THR

ALA

GLY

PRO

LYS

10

ASP

MET

VAL

GLY

PHE

ALA

ASN

LEU

GLY

11

ILE

LEU

HIS

VAL

THR

LYS

VAL

PHE

12

GLU

THR

LEU

GLU

ALA

ARG

MET

THR

GLU

ALA

13

CYS

ILE

ARG

GLY

TYR

ASN

PRO

GLY

LEU

14

VAL

HIS

SER

ASP

LEU

ALA

TYR

LEU

GLN

ALA

15

GLU

GLY

ASP

ARG

GLN

LEU

THR

ASP

16

ARG

GLU

LYS

GLU

ILE

ARG

GLN

ALA

17

VAL

GLN

THR

LYS

GLU

MET

ASP

LEU

SER

18

VAL

ARG

LEU

MET

PHE

THR

ALA

PHE

LEU

19

PRO

ASP

SER

THR

GLY

SER

PHE

THR

ARG

20

LEU

GLU

PRO

VAL

SER

ASP

ALA

ILE

TYR

21

ASP

SER

LYS

ALA

PRO

ASN

ALA

Samples:

sample_1: N-terminal domain of p50 subunit of NF-kappaB, [U-99% 15N], 0.45 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

CARA - data analysis

Relax - data analysis

NMR spectrometers:

Bruker Ascend 800 MHz