BMRB Entry 50811

Title:
NMR resonance assignment of the green kiwi fruit allergen Act d 8.0101
Deposition date:
2021-03-09
Original release date:
2021-06-25
Authors:
Zeindl, Ricarda; Tollinger, Martin
Citation:

Citation: Zeindl, Ricarda; Tollinger, Martin. "NMR resonance assignments of the PR-10 allergens Act c 8 and Act d 8 from golden and green kiwifruit"  Biomol. NMR Assignments 15, 367-371 (2021).
PubMed: 34106433

Assembly members:

Assembly members:
entity_1, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: kiwi fruit   Taxonomy ID: 3627   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Actinidia deliciosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b(+)

Data sets:
Data typeCount
13C chemical shifts668
15N chemical shifts150
1H chemical shifts980

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Act d 8.01011

Entities:

Entity 1, Act d 8.0101 156 residues - Formula weight is not available

1   GLYALAILETHRTYRASPMETGLUILEPRO
2   SERSERILESERALAGLULYSMETPHELYS
3   ALAPHEVALLEUASPGLYASPTHRILEILE
4   PROLYSALALEUPROHISALAILETHRGLY
5   VALGLNTHRLEUGLUGLYASPGLYGLYVAL
6   GLYTHRILELYSLEUTHRTHRPHEGLYGLU
7   GLYSERVALHISLYSSERVALLYSHISARG
8   ILEASPGLYLEUASPLYSGLUASNPHETHR
9   TYRSERTYRSERILEILEGLUGLYGLYALA
10   LEUASPVALPHEGLUSERILESERTYRHIS
11   ILELYSILEVALALATHRPROASPGLYGLY
12   CYSILECYSLYSASNARGSERILETYRTHR
13   PROLYSCYSASPALAGLNVALSERGLUGLU
14   GLUILELYSALAGLYLYSGLUARGALASER
15   GLYILEPHELYSLYSVALGLUALATYRLEU
16   LEUALAASNPROASPCYS

Samples:

sample_1: Act d 8.0101, [U-99% 15N], 0.5 mM; sodium phosphate 20 mM

sample_2: Act d 8.0101, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D (H)CC(CO)NH-TOCSYsample_2isotropicsample_conditions_1

Software:

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Agilent DD2 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks