BMRB Entry 50798

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50798

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Title:
GHR-TMD in DHPC micelles
Deposition date:
2021-03-02
Original release date:
2021-08-10
Authors:
Steinocher, Helena; Bugge, Katrine; Kragelund, Birthe
Citation:

Citation: Kassem, Noah; Araya-Secchi, Raul; Bugge, Katrine; Barclay, Abigail; Steinocher, Helena; Khondker, Adree; Wang, Yong; J. Lenard, Aneta; Burck, Jochen; Sahin, Cagla; S. Ulrich, Anne; Landreh, Michael; Cramer Pedersen, Martin; C. Rheinstadter, Maikel; Amstrup Pedersen, Per; Lindorff-Larsen, Kresten; Arleth, Lise; B. Kragelund, Birthe. "Order and disorder-An integrative structure of the full-length human growth hormone receptor"  Sci. Adv. 7, eabh3805-eabh3805 (2021).
PubMed: 34193419

Assembly members:

Assembly members:
entity_1, polymer, 39 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSQFTCEEDFYFPWLLIIIF GIFGLTVMLFVFLFSKQQR

Data sets:
Data typeCount
13C chemical shifts101
15N chemical shifts35
1H chemical shifts52

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TMD1

Entities:

Entity 1, TMD 39 residues - Formula weight is not available

1   GLYSERGLNPHETHRCYSGLUGLUASPPHE
2   TYRPHEPROTRPLEULEUILEILEILEPHE
3   GLYILEPHEGLYLEUTHRVALMETLEUPHE
4   VALPHELEUPHESERLYSGLNGLNARG

Samples:

sample_1: GHR-TMD, [U-13C; U-15N], 1 mM; DSS 1 mM; TCEP 2 mM; sodium azide 0.05 % v/v; potassium chloride 50 mM; sodium phosphate 20 mM; DHPC 210 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAHCsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks