BMRB Entry 50797

Name: Backbone assignment for yeast Yth1 zinc finger domain 4, 5 and C-terminal residues
Published: 2021-09-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50797

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment for yeast Yth1 zinc finger domain 4, 5 and C-terminal residues

Deposition date:

2021-03-01

Original release date:

2021-09-13

Authors:

Yu, Conny; Kumar, Ananthanarayanan; Rodriguez-Molina, Juan; Li, Xiaohan; Freund, Stefan; Passmore, Lori

Citation:

Citation: Kumar, Ananthanarayanan; Yu, Conny; Rodriguez-Molina, Juan; Li, Xiaohan; Freund, Stefan; Passmore, Lori. "Dynamics in Fip1 regulate eukaryotic mRNA 3' end processing" Genes Dev. 35, 1510-1526 (2021).
PubMed: 34593603

Assembly members:

Assembly members:
entity_1, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSMASKIPKCENYEMGF CPLGSSCPRRHIKKVFCQRY MTGFCPLGKDECDMEHPQFI IPDEGSKLRIKRDDEINTRK MDEEKERRLNAIINGEV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	240
15N chemical shifts	76
1H chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Yth1ZF45C	1

Entities:

Entity 1, Yth1ZF45C 97 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

MET

ALA

SER

LYS

ILE

2

PRO

LYS

CYS

GLU

ASN

TYR

GLU

MET

GLY

PHE

3

CYS

PRO

LEU

GLY

SER

CYS

PRO

ARG

4

HIS

ILE

LYS

VAL

PHE

CYS

GLN

ARG

TYR

5

MET

THR

GLY

PHE

CYS

PRO

LEU

GLY

LYS

ASP

6

GLU

CYS

ASP

MET

GLU

HIS

PRO

GLN

PHE

ILE

7

ILE

PRO

ASP

GLU

GLY

SER

LYS

LEU

ARG

ILE

8

LYS

ARG

ASP

GLU

ILE

ASN

THR

ARG

LYS

9

MET

ASP

GLU

LYS

GLU

ARG

LEU

ASN

10

ALA

ILE

ASN

GLY

GLU

VAL

Samples:

sample_1: Yth1ZF45C, [U-100% 15N], 100 uM; HEPES 50 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CAN)NH	sample_1	isotropic	sample_conditions_1
3D HN(COCA)NNH	sample_1	isotropic	sample_conditions_1
2D CON	sample_1	isotropic	sample_conditions_1
3D H(CA)CON	sample_1	isotropic	sample_conditions_1
3D H(CA)NCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.6.1 - collection, processing

NMRFAM-SPARKY v1.47 - chemical shift assignment, data analysis, peak picking

MddNMR - processing

MARS - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks