BMRB Entry 50795

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50795

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Title:
Backbone assignment for yeast Fip1 (1-226)
Deposition date:
2021-03-01
Original release date:
2021-09-13
Authors:
Yu, Conny; Kumar, Ananthanarayanan; Rodriguez-Molina, Juan; Li, Xiaohan; Freund, Stefan; Passmore, Lori
Citation:

Citation: Kumar, Ananthanarayanan; Yu, Conny; Rodriguez-Molina, Juan; Li, Xiaohan; Freund, Stefan; Passmore, Lori. "Dynamics in Fip1 regulate eukaryotic mRNA 3' end processing"  Genes Dev. 35, 1510-1526 (2021).
PubMed: 34593603

Assembly members:

Assembly members:
entity_1, polymer, 226 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSSSEDEDDKFLYGSDSELA LPSSKRSRDDEADAGASSNP DIVKRQKFDSPVEETPATAR DDRSDEDIYSDSSDDDSDSD LEVIISLGPDPTRLDAKLLD SYSTAATSSSKDVISVATDV SNTITKTSDERLITEGEANQ GVTATTVKATESDGNVPKAM TGSIDLDKEGIFDSVGITTI DPEVLKEKPWRQPGANLSDY FNYGFNEFTWMEYLHRQEKL QQDYNP

Data sets:
Data typeCount
13C chemical shifts549
15N chemical shifts188
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fip11

Entities:

Entity 1, Fip1 226 residues - Formula weight is not available

1   METSERSERSERGLUASPGLUASPASPLYS
2   PHELEUTYRGLYSERASPSERGLULEUALA
3   LEUPROSERSERLYSARGSERARGASPASP
4   GLUALAASPALAGLYALASERSERASNPRO
5   ASPILEVALLYSARGGLNLYSPHEASPSER
6   PROVALGLUGLUTHRPROALATHRALAARG
7   ASPASPARGSERASPGLUASPILETYRSER
8   ASPSERSERASPASPASPSERASPSERASP
9   LEUGLUVALILEILESERLEUGLYPROASP
10   PROTHRARGLEUASPALALYSLEULEUASP
11   SERTYRSERTHRALAALATHRSERSERSER
12   LYSASPVALILESERVALALATHRASPVAL
13   SERASNTHRILETHRLYSTHRSERASPGLU
14   ARGLEUILETHRGLUGLYGLUALAASNGLN
15   GLYVALTHRALATHRTHRVALLYSALATHR
16   GLUSERASPGLYASNVALPROLYSALAMET
17   THRGLYSERILEASPLEUASPLYSGLUGLY
18   ILEPHEASPSERVALGLYILETHRTHRILE
19   ASPPROGLUVALLEULYSGLULYSPROTRP
20   ARGGLNPROGLYALAASNLEUSERASPTYR
21   PHEASNTYRGLYPHEASNGLUPHETHRTRP
22   METGLUTYRLEUHISARGGLNGLULYSLEU
23   GLNGLNASPTYRASNPRO

Samples:

sample_1: Fip1, [U-100% 15N], 100 uM; HEPES 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CAN)NHsample_1isotropicsample_conditions_1
3D HN(COCA)NNHsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
3D H(CA)CONsample_1isotropicsample_conditions_1
3D H(CA)NCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6.1 - collection, processing

NMRFAM-SPARKY v1.47 - chemical shift assignment, data analysis, peak picking

MddNMR - processing

MARS - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks