BMRB Entry 50794

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50794

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Title:
NMR assignment of the Feline Immunodeficiency Virus p24 capsid
Deposition date:
2021-03-01
Original release date:
2021-06-13
Authors:
Long, Mathieu; Cantrelle, Francois-Xavier; Robert, Xavier; Boll, Emmanuelle; Sierra, Natalia; Gouet, Patrice; Hanoulle, Xavier; Alvarez, Guzman; Guillon, Christophe
Citation:

Citation: Long, Mathieu; Cantrelle, Francois-Xavier; Robert, Xavier; Boll, Emmanuelle; Sierra, Natalia; Gouet, Patrice; Hanoulle, Xavier; Alvarez, Guzman; Guillon, Christophe. "Identification of a Potential Inhibitor of the FIV p24 Capsid Protein and Characterization of Its Binding Site"  Biochemistry 60, 1896-1908 (2021).
PubMed: 34096272

Assembly members:

Assembly members:
entity_1, polymer, 213 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: FIV   Taxonomy ID: 11673   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus FIV

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPSET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TIQTVNGVPQYVALDPKMVS IFMEKAREGLGGEEVQLWFT AFSANLTPTDMATLIMAAPG CAADKEILDESLKQLTAEYD RTHPPDAPRPLPYFTAAEIM GIGLTQEQQAEARFAPARMQ CRAWYLEALGKLAAIKAKSP RAVQLRQGAKEDYSSFIDRL FAQIDQEQNTAEVKLYLKQS LSIANANADCKKAMSHLKPE STLEEKLRACQEI

Data sets:
Data typeCount
13C chemical shifts522
15N chemical shifts167
1H chemical shifts167

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FIV p241

Entities:

Entity 1, FIV p24 213 residues - Formula weight is not available

1   THRILEGLNTHRVALASNGLYVALPROGLN
2   TYRVALALALEUASPPROLYSMETVALSER
3   ILEPHEMETGLULYSALAARGGLUGLYLEU
4   GLYGLYGLUGLUVALGLNLEUTRPPHETHR
5   ALAPHESERALAASNLEUTHRPROTHRASP
6   METALATHRLEUILEMETALAALAPROGLY
7   CYSALAALAASPLYSGLUILELEUASPGLU
8   SERLEULYSGLNLEUTHRALAGLUTYRASP
9   ARGTHRHISPROPROASPALAPROARGPRO
10   LEUPROTYRPHETHRALAALAGLUILEMET
11   GLYILEGLYLEUTHRGLNGLUGLNGLNALA
12   GLUALAARGPHEALAPROALAARGMETGLN
13   CYSARGALATRPTYRLEUGLUALALEUGLY
14   LYSLEUALAALAILELYSALALYSSERPRO
15   ARGALAVALGLNLEUARGGLNGLYALALYS
16   GLUASPTYRSERSERPHEILEASPARGLEU
17   PHEALAGLNILEASPGLNGLUGLNASNTHR
18   ALAGLUVALLYSLEUTYRLEULYSGLNSER
19   LEUSERILEALAASNALAASNALAASPCYS
20   LYSLYSALAMETSERHISLEULYSPROGLU
21   SERTHRLEUGLUGLULYSLEUARGALACYS
22   GLNGLUILE

Samples:

sample_1: FIV p24, [U-13C; U-15N; U-2H], 120 uM; potassium phosphate 50 mM; sodium chloride 100 mM; DTT 3 mM; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D BEST TROSY HN(CO)CACBsample_1isotropicsample_conditions_1
3D BEST TROSY HNCACBsample_1isotropicsample_conditions_1
3D BEST TROSY HNCOsample_1isotropicsample_conditions_1
3D BEST TROSY HN(CA)COsample_1isotropicsample_conditions_1
TROSY (H)N(CA)NNHsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.5.1 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks