BMRB Entry 50790

Name: Triple Resonance Assignments of Human D-Dopachrome Tautomerase (MIF2)
Published: 2021-08-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50790

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Triple Resonance Assignments of Human D-Dopachrome Tautomerase (MIF2)

Deposition date:

2021-02-25

Original release date:

2021-08-17

Authors:

Chen, Emily; Lisi, George

Citation:

Citation: Chen, Emily; Reiss, Krystle; Shah, Dilip; Manjula, Ramu; Allen, Brandon; Murphy, Eva; Murphy, James; Batista, Victor; Bhandari, Vineet; Lolis, Elias; Lisi, George. "A Structurally Preserved Allosteric Site in the MIF Superfamily Affects Enzymatic Activity and CD74 Activation in D-dopachrome Tautomerase" J. Biol. Chem. 297, 101061-101061 (2021).
PubMed: 34384784

Assembly members:

Assembly members:
entity_1, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PFLELDTNLPANRVPAGLEK RLCAAAASILGKPADRVNVT VRPGLAMALSGSTEPCAQLS ISSIGVVGTAEDNRSHSAHF FEFLTKELALGQDRILIRFF PLESWQIGKIGTVMTFL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	186
15N chemical shifts	100
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DDT	1

Entities:

Entity 1, DDT 117 residues - Formula weight is not available

1

PRO

PHE

LEU

GLU

LEU

ASP

THR

ASN

LEU

PRO

2

ALA

ASN

ARG

VAL

PRO

ALA

GLY

LEU

GLU

LYS

3

ARG

LEU

CYS

ALA

SER

ILE

LEU

4

GLY

LYS

PRO

ALA

ASP

ARG

VAL

ASN

VAL

THR

5

VAL

ARG

PRO

GLY

LEU

ALA

MET

ALA

LEU

SER

6

GLY

SER

THR

GLU

PRO

CYS

ALA

GLN

LEU

SER

7

ILE

SER

ILE

GLY

VAL

GLY

THR

ALA

8

GLU

ASP

ASN

ARG

SER

HIS

SER

ALA

HIS

PHE

9

PHE

GLU

PHE

LEU

THR

LYS

GLU

LEU

ALA

LEU

10

GLY

GLN

ASP

ARG

ILE

LEU

ILE

ARG

PHE

11

PRO

LEU

GLU

SER

TRP

GLN

ILE

GLY

LYS

ILE

12

GLY

THR

VAL

MET

THR

PHE

LEU

Samples:

sample_1: DDT, [U-13C; U-15N; U-2H], 600 uM; TRIS 20 mM; sodium chloride 20 mM

sample_conditions_1: ionic strength: 0.04 M; pH: 7.4; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMRPipe - processing

PINE - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz
Bruker AVANCE III 850 MHz

UNP	Q53Y51
AlphaFold	Q53Y51