BMRB Entry 50781

Title:
Backbone 1H, 13C, and 15N chemical shift assignments for ClpX residues 1-55
Deposition date:
2021-02-19
Original release date:
2022-04-15
Authors:
Vos, Margaret; Peti, Wolfgang; Page, Rebecca
Citation:

Citation: Vos, Margaret; Piraino, Benjamin; LaBreck, Christopher; Rahmani, Negar; Trebino, Catherine; Schoenle, Marta; Peti, Wolfgang; Camberg, Jodi; Page, Rebecca. "Degradation of the E. coli antitoxin MqsA by the proteolytic complex ClpXP is regulated by zinc occupancy and oxidation"  J. Biol. Chem. 298, 101557-101557 (2022).
PubMed: 34974059

Assembly members:

Assembly members:
entity_1, polymer, 57 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: RP1B

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts111
15N chemical shifts53
1H chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ClpX Residues 1-55 Dimer, Chain 11
2ClpX Residues 1-55 Dimer, Chain 21
3Zinc Ion, 12
4Zinc Ion, 22

Entities:

Entity 1, ClpX Residues 1-55 Dimer, Chain 1 57 residues - Formula weight is not available

GH are a cloning artifacts; ClpX sequence starts with MTD

1   GLYHISMETTHRASPLYSARGLYSASPGLY
2   SERGLYLYSLEULEUTYRCYSSERPHECYS
3   GLYLYSSERGLNHISGLUVALARGLYSLEU
4   ILEALAGLYPROSERVALTYRILECYSASP
5   GLUCYSVALASPLEUCYSASNASPILEILE
6   ARGGLUGLUILELYSGLUVAL

Entity 2, Zinc Ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: ClpX Residues 1-55, [U-99% 13C; U-99% 15N], 800 uM; TCEP 0.5 mM; sodium chloride 50 mM; sodium phosphate 20 mM; ZnSO4 500 uM

sample_2: ClpX Residues 1-55, [U-99% 15N], 200 uM; TCEP 0.5 mM; sodium chloride 50 mM; sodium phosphate 20 mM; ZnSO4 500 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCCONHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.1.1 - collection, data analysis

CARA v1.9 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Related Database Links:

NCBI P0A6H1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks