BMRB Entry 50779

Name: Structure and topology of DWORF-P15A in bicelles by oriented solid-state NMR
Published: 2021-12-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50779

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure and topology of DWORF-P15A in bicelles by oriented solid-state NMR

Deposition date:

2021-02-19

Original release date:

2021-12-14

Authors:

Uddigiri, Venkateswara Reddy; Weber, Daniel; Veglia, Gianluigi

Citation:

Citation: Reddy, U Venkateswara; Weber, Daniel; Wang, Songlin; Larsen, Erik; Gopinath, Tata; De Simone, Alfonso; Robia, Seth; Veglia, Gianluigi. "A kink in DWORF helical structure controls the activation of the sarcoplasmic reticulum Ca 2+-ATPase" Structure 30, 360-370 (2022).
PubMed: 34875216

Assembly members:

Assembly members:
entity_1, polymer, 36 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMAL-c2X

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AMAEKAGSTFSHLLVAILLL IGWIVGCIIMIYVVFS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
dipolar_couplings
- dipolar_couplings_1

Data type	Count
15N chemical shifts	19
dipolar coupling values	19

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DWORF-P15A	1

Entities:

Entity 1, DWORF-P15A 36 residues - Formula weight is not available

1

ALA

MET

ALA

GLU

LYS

ALA

GLY

SER

THR

PHE

2

SER

HIS

LEU

VAL

ALA

ILE

LEU

3

ILE

GLY

TRP

ILE

VAL

GLY

CYS

ILE

MET

4

ILE

TYR

VAL

PHE

SER

Samples:

sample_1: DWORF-P15A alone in bicelles, [U-100% 15N], 1.8 mM; HEPES 20 mM; potassium chloride 20 mM; magnesium chloride 5 mM; ethylene glycol-bis(b-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) 5 mM; sodium azide 0.02%; glycerol 2.5%; DTT 5 mM; 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) 267 mM; 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) 67 mM; 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) 74 mM; 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (14:0 PE-DTPA) 6 mM; ytterbium chloride 5 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N SE-SAMPI4	sample_1	anisotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - data analysis

VNMRj - collection

NMRPipe - processing

NMR spectrometers:

Varian VXRS 600 MHz