BMRB Entry 50778

Title:
Structure and topology of DWORF in bicelles by oriented solid-state NMR
Deposition date:
2021-02-19
Original release date:
2021-06-25
Authors:
Uddigiri, Venkateswara Reddy; Weber, Daniel; Veglia, Gianluigi
Citation:

Citation: Reddy, U Venkateswara; Weber, Daniel; Wang, Songlin; Larsen, Erik; Gopinath, Tata; De Simone, Alfonso; Robia, Seth; Veglia, Gianluigi. "A kink in DWORF helical structure controls the activation of the sarcoplasmic reticulum Ca 2+-ATPase"  Structure 30, 360-370 (2022).
PubMed: 34875216

Assembly members:

Assembly members:
entity_1, polymer, 36 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMAL-c2X

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AMAEKAGSTFSHLLVPILLL IGWIVGCIIMIYVVFS

Data sets:
Data typeCount
15N chemical shifts28
dipolar coupling values28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DWORF1

Entities:

Entity 1, DWORF 36 residues - Formula weight is not available

1   ALAMETALAGLULYSALAGLYSERTHRPHE
2   SERHISLEULEUVALPROILELEULEULEU
3   ILEGLYTRPILEVALGLYCYSILEILEMET
4   ILETYRVALVALPHESER

Samples:

sample_1: DWORF alone in bicelles, [U-100% 15N], 2.5 mM; HEPES 20 mM; potassium chloride 20 mM; magnesium chloride 5 mM; ethylene glycol-bis(b-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) 5 mM; sodium azide 0.02%; glycerol 2.5%; DTT 5 mM; 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) 267 mM; 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) 67 mM; 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) 74 mM; 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (14:0 PE-DTPA) 6 mM; ytterbium chloride 5 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N SE-SAMPI4sample_1anisotropicsample_conditions_1

Software:

NMRFAM-SPARKY - data analysis

VNMRj - collection

NMRPipe - processing

NMR spectrometers:

  • Varian VXRS 600 MHz