BMRB Entry 50776

Name: Substrate sequence determinants governing the specificity of ClpS1 in plant chloroplasts
Published: 2021-06-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50776

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Substrate sequence determinants governing the specificity of ClpS1 in plant chloroplasts

Deposition date:

2021-02-18

Original release date:

2021-06-14

Authors:

Aguilar Lucero, Dianela; Cantoia, Alejo; Sanchez-Lopez, Carolina; Binolfi, Andres; Mogk, Axel; Ceccarelli, Eduardo; Rosano, German

Citation:

Citation: Aguilar Lucero, Dianela; Cantoia, Alejo; Sanchez-Lopez, Carolina; Binolfi, Andres; Mogk, Axel; Ceccarelli, Eduardo; Rosano, German. "Structural features of the plant N-recognin ClpS1 and sequence determinants in its targets that govern substrate selection" FEBS Lett. 595, 1525-1541 (2021).
PubMed: 33792910

Assembly members:

Assembly members:
entity_1, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETGEXCT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AAALGKGGGVLDKPIIEKTT PGRESEFDLRKSKKIAPPYR VILHNDNFNKREYVVQVLMK VIPGMTVDNAVNIMQEAHIN GLAVVIVCAQADAEQHCMQL RGNGLLSSVEPDGGGCAEFL VPR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	339
15N chemical shifts	105
1H chemical shifts	105

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ClpS1	1

Entities:

Entity 1, ClpS1 123 residues - Formula weight is not available

First Ala residue comes from the cloning procedure and does not belong to the protein's original sequence.

1

ALA

LEU

GLY

LYS

GLY

VAL

2

LEU

ASP

LYS

PRO

ILE

GLU

LYS

THR

3

PRO

GLY

ARG

GLU

SER

GLU

PHE

ASP

LEU

ARG

4

LYS

SER

LYS

ILE

ALA

PRO

TYR

ARG

5

VAL

ILE

LEU

HIS

ASN

ASP

ASN

PHE

ASN

LYS

6

ARG

GLU

TYR

VAL

GLN

VAL

LEU

MET

LYS

7

VAL

ILE

PRO

GLY

MET

THR

VAL

ASP

ASN

ALA

8

VAL

ASN

ILE

MET

GLN

GLU

ALA

HIS

ILE

ASN

9

GLY

LEU

ALA

VAL

ILE

VAL

CYS

ALA

GLN

10

ALA

ASP

ALA

GLU

GLN

HIS

CYS

MET

GLN

LEU

11

ARG

GLY

ASN

GLY

LEU

SER

VAL

GLU

12

PRO

ASP

GLY

CYS

ALA

GLU

PHE

LEU

13

VAL

PRO

ARG

Samples:

sample_1: ClpS1, [U-100% 13C; U-100% 15N], 800 uM; phosphate buffer 20 mM; NaCl 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.5.5 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks