BMRB Entry 50766

Name: Backbone resonance assignments of LINE-1 retrotransposable element ORF1 protein N-terminal region.
Published: 2021-03-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50766

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments of LINE-1 retrotransposable element ORF1 protein N-terminal region.

Deposition date:

2021-02-16

Original release date:

2021-03-03

Authors:

Naik, Mandar; Fawzi, Nicolas; Jogl, Gerwald; Sedivy, John

Citation:

Citation: Newton, Jocelyn; Naik, Mandar; Li, Grace; Murphy, Eileen; Fawzi, Nicolas; Sedivy, John; Jogl, Gerwald. "Phase separation of the LINE-1 ORF1 protein is mediated by the N-terminus and coiled-coil domain." Biophys. J. 120, 2181-2191 (2021).
PubMed: 33798566

Assembly members:

Assembly members:
entity_1, polymer, 61 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pEt26

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGKKQNRKTGNSKTQSASPP PKERSSSPATEQSWMENDFD ELREEGFRRSNYSLEHHHHH H

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	170
15N chemical shifts	54
1H chemical shifts	54

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ORF1 1-53	1

Entities:

Entity 1, ORF1 1-53 61 residues - Formula weight is not available

Residue 54 to 61 are non-native purification tag

1

MET

GLY

LYS

GLN

ASN

ARG

LYS

THR

GLY

2

ASN

SER

LYS

THR

GLN

SER

ALA

SER

PRO

3

PRO

LYS

GLU

ARG

SER

PRO

ALA

THR

4

GLU

GLN

SER

TRP

MET

GLU

ASN

ASP

PHE

ASP

5

GLU

LEU

ARG

GLU

GLY

PHE

ARG

SER

6

ASN

TYR

SER

LEU

GLU

HIS

7

HIS

Samples:

sample_1: ORF1 1-53, [U-99% 15N], 0.51 mM; ORF1 1-53, [U-99% 13C; U-99% 15N], 0.7 mM; MES 20 mM; NaCl 200 mM; DTT 1 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.7 - collection, processing

SPARKY v3.115 - data analysis

NMR spectrometers:

Bruker AVANCE III 850 MHz
Bruker AVANCE NEO 600 MHz

UNP	Q9UN81
AlphaFold	Q15605