BMRB Entry 50759

Title:
Backbone resonance assignment of Phosphorylated C-terminal intracellular domain of Tir effector from Escherichia coli O127:H6
Deposition date:
2021-02-10
Original release date:
2024-02-14
Authors:
Vieira, Marta
Citation:

Citation: Vieira, Marta; Hernandez, Guillem; Zhong, Qiyun; Arbesu, Miguel; Veloso, Tiago; Gomes, Tiago; Martins, Maria; Monteiro, Hugo; Frazao, Carlos; Frankel, Gad; Zanzoni, Andreas; Cordeiro, Tiago. "The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection"  Commun. Biol. 7, 179-179 (2024).
PubMed: 38351154

Assembly members:

Assembly members:
entity_1, polymer, 173 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHTP8

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts160
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pC-Tir1

Entities:

Entity 1, pC-Tir 173 residues - Formula weight is not available

N-Terminal: two residues (GP) from HRV-3C cleavage sequence C-Terminal: Streptag (WSHPQFEK) Phosphorylated tyrosine residues Y454, Y474, Y483 and Y511.

1   GLYPROARGARGASNGLNPROALAGLUGLN
2   THRTHRTHRTHRTHRTHRHISTHRVALVAL
3   GLNGLNGLNTHRGLYGLYASNTHRPROALA
4   GLNGLYGLYTHRASPALATHRARGALAGLU
5   ASPALASERLEUASNARGARGASPSERGLN
6   GLYSERVALALASERTHRHISTRPSERASP
7   SERSERSERGLUVALVALASNPROPTRALA
8   GLUVALGLYGLYALAARGASNSERLEUSER
9   ALAHISGLNPROGLUGLUHISILEPTRASP
10   GLUVALALAALAASPPROGLYPTRSERVAL
11   ILEGLNASNPHESERGLYSERGLYPROVAL
12   THRGLYARGLEUILEGLYTHRPROGLYGLN
13   GLYILEGLNSERTHRPTRALALEULEUALA
14   ASNSERGLYGLYLEUARGLEUGLYMETGLY
15   GLYLEUTHRSERGLYGLYGLUSERALAVAL
16   SERSERVALASNALAALAPROTHRPROGLY
17   PROVALARGPHEVALTRPSERHISPROGLN
18   PHEGLULYS

Samples:

sample_1: Phosphorylated C-Tir, [U-13C; U-15N], 991.1 uM; D2O, [U-2H], 8 % v/v; sodium chloride 150 mM; HEPES 20 mM; magnesium chloride 12 mM; Adenosine triphosphate 5.5 mM; DSS 20 uM

sample_conditions_1: ionic strength: 150 mM; pH: 6.80; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CARA v1.9.1 - chemical shift assignment, data analysis

TOPSPIN - NMR data acquisition and spectra processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP B7UM99

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks