BMRB Entry 50747

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50747

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Title:
1H, 13C, 15N backbone NMR resonance assignments of Psb28 in complex with the C-terminal peptide of CP47
Deposition date:
2021-02-04
Original release date:
2021-04-15
Authors:
Arnolds, Oliver; Stoll, Raphael
Citation:

Citation: Zabret, Jure; Bohn, Stefan; Schuller, Sandra; Arnolds, Oliver; Moller, Madeline; Meier-Credo, Jakob; Liauw, Pasqual; Chan, Aaron; Tajkhorshid, Emad; Langer, Julian; Stoll, Raphael; Krieger-Liszkay, Anja; Engel, Benjamin; Rudack, Till; Schuller, Jan; Nowaczyk, Marc. "Structural insights into photosystem II assembly"  Nat. Plants 7, 524-538 (2021).
PubMed: 33846594

Assembly members:

Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available
entity_2, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermosynechococcus elongatus   Taxonomy ID: 146786   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pIVEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AHMGAMAEIQFIRGINEEVV PDVRLTRARDGSSGQAMFYF DNPKIVQEGNLEVTGMYMVD EEGEIVTRDVNAKFINGQPV AIEATYTMRSPQEWDRFIRF MDRYAASHGLGFQKSENSPG GG
entity_2: SGIDPELSPEQVEWGFYQKV

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts117
1H chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Psb281
2CP47 C-terminus2

Entities:

Entity 1, Psb28 122 residues - Formula weight is not available

1   ALAHISMETGLYALAMETALAGLUILEGLN
2   PHEILEARGGLYILEASNGLUGLUVALVAL
3   PROASPVALARGLEUTHRARGALAARGASP
4   GLYSERSERGLYGLNALAMETPHETYRPHE
5   ASPASNPROLYSILEVALGLNGLUGLYASN
6   LEUGLUVALTHRGLYMETTYRMETVALASP
7   GLUGLUGLYGLUILEVALTHRARGASPVAL
8   ASNALALYSPHEILEASNGLYGLNPROVAL
9   ALAILEGLUALATHRTYRTHRMETARGSER
10   PROGLNGLUTRPASPARGPHEILEARGPHE
11   METASPARGTYRALAALASERHISGLYLEU
12   GLYPHEGLNLYSSERGLUASNSERPROGLY
13   GLYGLY

Entity 2, CP47 C-terminus 20 residues - Formula weight is not available

1   SERGLYILEASPPROGLULEUSERPROGLU
2   GLNVALGLUTRPGLYPHETYRGLNLYSVAL

Samples:

sample_1: Psb28, [U-100% 15N], 0.138 mM; CP47 C-terminus 0.3 mM; Tris/HCl 20 mM; NaN3 0.02%

sample_2: Psb28, [U-100% 13C; U-100% 15N], 1 mM; CP47 C-terminus 4 mM; Tris/HCl 20 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 20 mM; pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
1D 1Hsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCE III 700 MHz
  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks