BMRB Entry 50741

Title:
Backbone 1H, 15N and 13C chemical shift assignments for HEV ORF1 peptide [1622-1647] (isolate G3-HEV83-2-27) in 20% TFE-d2
Deposition date:
2021-01-30
Original release date:
2023-02-07
Authors:
Cantrelle, Francois-Xavier; Gouttenoire, Jerome; Hanoulle, Xavier
Citation:

Citation: Oechslin, Noemie; Da Silva, Nathalie; Szkolnicka, Dagmara; Cantrelle, Francois-Xavier; Hanoulle, Xavier; Moradpour, Darius; Gouttenoire, Jerome. "Hepatitis E virus RNA-dependent RNA polymerase is involved in RNA replication and infectious particle production"  Hepatology 75, 170-181 (2022).
PubMed: 34387882

Assembly members:

Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Hepatitis E virus   Taxonomy ID: 12461   Superkingdom: Viruses   Kingdom: not available   Genus/species: Orthohepevirus Orthohepevirus A

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHisSUMO-pORF1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KNWGPGPERAEQLRLAVCDF LRGLTN

Data sets:
Data typeCount
13C chemical shifts76
15N chemical shifts26
1H chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ORF1 peptide1

Entities:

Entity 1, ORF1 peptide 26 residues - Formula weight is not available

Sequence numbering according to the full-length HEV ORF1 polyprotein

1   LYSASNTRPGLYPROGLYPROGLUARGALA
2   GLUGLNLEUARGLEUALAVALCYSASPPHE
3   LEUARGGLYLEUTHRASN

Samples:

sample_1: HEV ORF1 peptide 1622-1647, [U-100% 13C; U-100% 15N], 280 uM; sodium phosphate 50 mM; sodium chloride 150 mM; THP 2 mM; TFE, [U-99% 2H], 20%; TSP 100 uM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.6 - collection

NMRPipe - processing

qMDD - processing

NMRFAM-SPARKY v3.190 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE NEO 900 MHz

Related Database Links:

GB AB740232

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks