BMRB Entry 50741

Name: Backbone 1H, 15N and 13C chemical shift assignments for HEV ORF1 peptide [1622-1647] (isolate G3-HEV83-2-27) in 20% TFE-d2
Published: 2023-02-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50741

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N and 13C chemical shift assignments for HEV ORF1 peptide [1622-1647] (isolate G3-HEV83-2-27) in 20% TFE-d2

Deposition date:

2021-01-30

Original release date:

2023-02-07

Authors:

Cantrelle, Francois-Xavier; Gouttenoire, Jerome; Hanoulle, Xavier

Citation:

Citation: Oechslin, Noemie; Da Silva, Nathalie; Szkolnicka, Dagmara; Cantrelle, Francois-Xavier; Hanoulle, Xavier; Moradpour, Darius; Gouttenoire, Jerome. "Hepatitis E virus RNA-dependent RNA polymerase is involved in RNA replication and infectious particle production" Hepatology 75, 170-181 (2022).
PubMed: 34387882

Assembly members:

Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Hepatitis E virus Taxonomy ID: 12461 Superkingdom: Viruses Kingdom: not available Genus/species: Orthohepevirus Orthohepevirus A

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHisSUMO-pORF1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KNWGPGPERAEQLRLAVCDF LRGLTN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	76
15N chemical shifts	26
1H chemical shifts	51

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ORF1 peptide	1

Entities:

Entity 1, ORF1 peptide 26 residues - Formula weight is not available

Sequence numbering according to the full-length HEV ORF1 polyprotein

1

LYS

ASN

TRP

GLY

PRO

GLY

PRO

GLU

ARG

ALA

2

GLU

GLN

LEU

ARG

LEU

ALA

VAL

CYS

ASP

PHE

3

LEU

ARG

GLY

LEU

THR

ASN

Samples:

sample_1: HEV ORF1 peptide 1622-1647, [U-100% 13C; U-100% 15N], 280 uM; sodium phosphate 50 mM; sodium chloride 150 mM; THP 2 mM; TFE, [U-99% 2H], 20%; TSP 100 uM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.6 - collection

NMRPipe - processing

qMDD - processing

NMRFAM-SPARKY v3.190 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE NEO 900 MHz