BMRB Entry 50740

Title:
Backbone assignments of the N-terminal region of the partitioning protein IncC1 from the plasmid RK2
Deposition date:
2021-01-29
Original release date:
2021-04-16
Authors:
Ur-Rehman, Fayyaz; Jeeves, Mark; Hyde, Eva
Citation:

Citation: Ur-Rehman, Fayyaz; Jeeves, Mark; Hyde, Eva. "Backbone assignments, and effect of Asn deamidation, of the N-terminal region of the partitioning protein IncC1 from the plasmid RK2"  Biomol. NMR Assignments ., .-. (2021).

Assembly members:

Assembly members:
entity_1, polymer, 128 residues, 13146 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET28a

Data sets:
Data typeCount
13C chemical shifts354
15N chemical shifts127
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IncC1 NTD1

Entities:

Entity 1, IncC1 NTD 128 residues - 13146 Da.

-22M to 0F is the non-native affinity tag. The native sequence starts at 1M. This is the N-terminal of IncC1, which is not found in IncC2

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   SERGLUPHEMETGLYVALILEHISGLUGLU
4   THRALATYRARGLYSPROVALPROGLYGLY
5   ASPPROGLYALAGLYSERGLYALAALAASP
6   HISARGASPSERALAGLYARGLEUSERARG
7   TRPGLUALATHRGLYASPVALARGASNVAL
8   ALAGLYTHRASPGLNGLYARGSERVALALA
9   SERGLYALASERARGVALGLYARGVALARG
10   GLYGLNGLULEUALAARGGLYVALARGALA
11   GLYASNGLYGLYSERALAGLYTHRSERGLY
12   VALHISARGPROGLUVALGLYSERGLYARG
13   GLNGLULYSTHRGLYASNGLNTHR

Samples:

sample_1: IncC1 NTD, [U-100% 13C; U-100% 15N], 300 uM; D2O, [U-2H], 10%; H2O 90%; sodium phosphate 10 mM; sodium chloride 150 mM; EDTA 0.1 mM

sample_conditions_1: ionic strength: 0.173 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
CONsample_1isotropicsample_conditions_1
(H)CBCACONsample_1isotropicsample_conditions_1
(H)CBCACOsample_1isotropicsample_conditions_1
HNNsample_1isotropicsample_conditions_1
HNCNsample_1isotropicsample_conditions_1
(H)CANCOsample_1isotropicsample_conditions_1

Software:

MddNMR - processing

NMRPipe - processing

ANALYSIS v2.0 - data analysis

TALOS-N - N Order parameter, secondary structure propensity

DANGLE - secondary structure propensity

xwinnmr - collection

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 900 MHz

Related Database Links:

UNP P07673-1
AlphaFold P07673

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks