BMRB Entry 50740

Name: Backbone assignments of the N-terminal region of the partitioning protein IncC1 from the plasmid RK2
Published: 2021-04-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50740

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of the N-terminal region of the partitioning protein IncC1 from the plasmid RK2

Deposition date:

2021-01-29

Original release date:

2021-04-16

Authors:

Ur-Rehman, Fayyaz; Jeeves, Mark; Hyde, Eva

Citation:

Citation: Ur-Rehman, Fayyaz; Jeeves, Mark; Hyde, Eva. "Backbone assignments, and effect of Asn deamidation, of the N-terminal region of the partitioning protein IncC1 from the plasmid RK2" Biomol. NMR Assignments ., .-. (2021).

Assembly members:

Assembly members:
entity_1, polymer, 128 residues, 13146 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH SEFMGVIHEETAYRKPVPGG DPGAGSGAADHRDSAGRLSR WEATGDVRNVAGTDQGRSVA SGASRVGRVRGQELARGVRA GNGGSAGTSGVHRPEVGSGR QEKTGNQT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	354
15N chemical shifts	127
1H chemical shifts	121

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IncC1 NTD	1

Entities:

Entity 1, IncC1 NTD 128 residues - 13146 Da.

-22M to 0F is the non-native affinity tag. The native sequence starts at 1M. This is the N-terminal of IncC1, which is not found in IncC2

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

SER

GLU

PHE

MET

GLY

VAL

ILE

HIS

GLU

4

THR

ALA

TYR

ARG

LYS

PRO

VAL

PRO

GLY

5

ASP

PRO

GLY

ALA

GLY

SER

GLY

ALA

ASP

6

HIS

ARG

ASP

SER

ALA

GLY

ARG

LEU

SER

ARG

7

TRP

GLU

ALA

THR

GLY

ASP

VAL

ARG

ASN

VAL

8

ALA

GLY

THR

ASP

GLN

GLY

ARG

SER

VAL

ALA

9

SER

GLY

ALA

SER

ARG

VAL

GLY

ARG

VAL

ARG

10

GLY

GLN

GLU

LEU

ALA

ARG

GLY

VAL

ARG

ALA

11

GLY

ASN

GLY

SER

ALA

GLY

THR

SER

GLY

12

VAL

HIS

ARG

PRO

GLU

VAL

GLY

SER

GLY

ARG

13

GLN

GLU

LYS

THR

GLY

ASN

GLN

THR

Samples:

sample_1: IncC1 NTD, [U-100% 13C; U-100% 15N], 300 uM; D2O, [U-2H], 10%; H2O 90%; sodium phosphate 10 mM; sodium chloride 150 mM; EDTA 0.1 mM

sample_conditions_1: ionic strength: 0.173 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
CON	sample_1	isotropic	sample_conditions_1
(H)CBCACON	sample_1	isotropic	sample_conditions_1
(H)CBCACO	sample_1	isotropic	sample_conditions_1
HNN	sample_1	isotropic	sample_conditions_1
HNCN	sample_1	isotropic	sample_conditions_1
(H)CANCO	sample_1	isotropic	sample_conditions_1

Software:

MddNMR - processing

NMRPipe - processing

ANALYSIS v2.0 - data analysis

TALOS-N - N Order parameter, secondary structure propensity

DANGLE - secondary structure propensity

xwinnmr - collection

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 900 MHz

UNP	P07673-1
AlphaFold	P07673