BMRB Entry 50739

Title:
Backbone 1H, 13C, and 15N Chemical Shift assignments for the +7K+12D mutant of hnRNPA1-LCD
Deposition date:
2021-01-29
Original release date:
2022-04-15
Authors:
Borcherds, Wade
Citation:

Citation: Bremer, Anne; Farag, Mina; Borcherds, Wade; Peran, Ivan; Martin, Erik; Pappu, Rohit; Mittag, Tanja. "Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains"  Nat. Chem. 14, 196-207 (2022).
PubMed: 34931046

Assembly members:

Assembly members:
entity_1, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDest17

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts128
1H chemical shifts128

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Low complexity domain (LCD)1

Entities:

Entity 1, Low complexity domain (LCD) 137 residues - Formula weight is not available

G1 to F137

1   GLYSERMETALASERALAASPSERSERGLN
2   ARGASPARGASPASPLYSGLYASNPHEGLY
3   ASPGLYARGGLYGLYGLYPHEGLYGLYASN
4   ASPASNPHEGLYARGGLYGLYASNPHESER
5   ASPARGGLYGLYPHEGLYGLYSERARGGLY
6   ASPGLYLYSTYRGLYGLYASPGLYASPLYS
7   TYRASNGLYPHEGLYASNASPGLYLYSASN
8   PHEGLYGLYGLYGLYSERTYRASNASPPHE
9   GLYASNTYRASNASNGLNSERSERASNPHE
10   ASPPROMETLYSGLYGLYASNPHELYSASP
11   ARGSERSERGLYPROTYRASPLYSGLYGLY
12   GLNTYRPHEALALYSPROARGASNGLNGLY
13   GLYTYRGLYGLYSERSERSERSERLYSSER
14   TYRGLYSERASPARGARGPHE

Samples:

sample_1: +7K+12D hnRNPA1 LCD, [U-99% 13C; U-99% 15N], 157.5 uM; DSS 20 uM; D2O 10%; HEPES 20 mM; sodium chloride 150 mM

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D NH(CA)NNHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0 - collection

NMRViewJ v9.2 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks