BMRB Entry 50737

Name: Smad4 interdomain linker
Published: 2021-09-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50737

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Smad4 interdomain linker

Deposition date:

2021-01-27

Original release date:

2021-09-14

Authors:

Gomes, Tiago

Citation:

Citation: Gomes, Tiago; Martin-Malpartida, Pau; Ruiz, Lidia; Aragona, Eric; Cordeiro, Tiago; Macias, Maria. "Conformational landscape of multidomain SMAD proteins" Comp. Struc. Biotech. J. 19, 5210-5224 (2021).
PubMed: 34630939

Assembly members:

Assembly members:
entity_1, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMIDLSGLTLQSNAPSSMM VKDEYVHDFEGQPSLSTEGH SIQTIQHPPSNRASTETYST PALLAPSESNATSTANFPNI PVASTSQPASILGGSHSEGL LQIASGPQPGQQQNGFTGQP ATYHHNSTTTWTGS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	257
15N chemical shifts	95
1H chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Smad4linker	1

Entities:

Entity 1, Smad4linker 134 residues - Formula weight is not available

1

GLY

ALA

MET

ILE

ASP

LEU

SER

GLY

LEU

THR

2

LEU

GLN

SER

ASN

ALA

PRO

SER

MET

3

VAL

LYS

ASP

GLU

TYR

VAL

HIS

ASP

PHE

GLU

4

GLY

GLN

PRO

SER

LEU

SER

THR

GLU

GLY

HIS

5

SER

ILE

GLN

THR

ILE

GLN

HIS

PRO

SER

6

ASN

ARG

ALA

SER

THR

GLU

THR

TYR

SER

THR

7

PRO

ALA

LEU

ALA

PRO

SER

GLU

SER

ASN

8

ALA

THR

SER

THR

ALA

ASN

PHE

PRO

ASN

ILE

9

PRO

VAL

ALA

SER

THR

SER

GLN

PRO

ALA

SER

10

ILE

LEU

GLY

SER

HIS

SER

GLU

GLY

LEU

11

LEU

GLN

ILE

ALA

SER

GLY

PRO

GLN

PRO

GLY

12

GLN

ASN

GLY

PHE

THR

GLY

GLN

PRO

13

ALA

THR

TYR

HIS

ASN

SER

THR

14

TRP

THR

GLY

SER

Samples:

sample_1: s4linker, [U-100% 13C; U-100% 15N], 400 uM

sample_conditions_1: ionic strength: 0.15 M; pH: 7; pressure: 1 atm; temperature: 275 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
HNCACONH	sample_1	isotropic	sample_conditions_1
HNCANH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks