BMRB Entry 50736

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50736

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Title:
Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease
Deposition date:
2021-01-27
Original release date:
2021-05-18
Authors:
Tzeng, Shiou-Ru
Citation:

Citation: Tzeng, Shiou-Ru; Tseng, Yin-Chu; Lin, Chien-Chu; Hsu, Chia-Ying; Huang, Shing-Jong; Kuo, Yi-Ting; Chang, Chung-I. "Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease"  elife 10, e64056-e64056 (2021).
PubMed: 33929321

Assembly members:

Assembly members:
entity_1, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-Ig2D5-S20

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ASSHATRQLSGLKIHSNLYH

Data sets:
Data typeCount
13C chemical shifts31
15N chemical shifts17
1H chemical shifts17

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sul20 in-NTD bound state1

Entities:

Entity 1, Sul20 in-NTD bound state 20 residues - Formula weight is not available

1   ALASERSERHISALATHRARGGLNLEUSER
2   GLYLEULYSILEHISSERASNLEUTYRHIS

Samples:

sample_1: Sul20 in-NTD bound state, [U-98% 15N], 0.05 mM; Sul20 in-NTD bound state, [U-99% 13C; U-99% 15N], 0.3 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker Bruker AVIII-800 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks