BMRB Entry 5073

Name: Solution structure of the monomeric variant of the chemokine MIP-1beta
Published: 2003-01-07

Click here to enlarge.

PDB ID: 1je4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5073
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the monomeric variant of the chemokine MIP-1beta

Deposition date:

2001-07-09

Original release date:

2003-01-07

Authors:

Kim, S.; Jao, S.; Laurence, J.; LiWang, P.

Citation:

Citation: Kim, S.; Jao, S.; Laurence, J.; LiWang, P.. "Structural Comparison of Monomeric Variants of the Chemokine MIP-1beta having Differing Ability to bind the Receptor CCR5" Biochemistry 40, 10782-10791 (2001).
PubMed: 11535053

Assembly members:

Assembly members:
MIP-1 beta, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MIP-1 beta: APMGSDPPTACCASYTARKL PRNFVVDYYETSSLCSQPAV VFQTKRSKQVCADPSESWVQ EYVYDLELN

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	190
15N chemical shifts	62
1H chemical shifts	376

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MIP-1-beta	1

Entities:

Entity 1, MIP-1-beta 69 residues - Formula weight is not available

1

ALA

PRO

MET

GLY

SER

ASP

PRO

THR

ALA

2

CYS

ALA

SER

TYR

THR

ALA

ARG

LYS

LEU

3

PRO

ARG

ASN

PHE

VAL

ASP

TYR

GLU

4

THR

SER

LEU

CYS

SER

GLN

PRO

ALA

VAL

5

VAL

PHE

GLN

THR

LYS

ARG

SER

LYS

GLN

VAL

6

CYS

ALA

ASP

PRO

SER

GLU

SER

TRP

VAL

GLN

7

GLU

TYR

VAL

TYR

ASP

LEU

GLU

LEU

ASN

Samples:

sample_1: MIP-1 beta, [U-15N; U-13C], 1 – 2 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 20 mM; pH: 2.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
HNHA	not available	not available	not available

Software:

NMRPipe - processing

CNS v1.0 - structure solution

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1HUM 1HUN 1JE4
EMBL	CAA34291 CAA37723 CAG46916
GenBank	AAA51576 AAA57256 AAI07434 AAX07292 AAX07305
REF	NP_002975
SWISS-PROT	P13236