BMRB Entry 50725

Name: backbone assignments of SARS-CoV-2 Nsp9
Published: 2021-02-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50725

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

backbone assignments of SARS-CoV-2 Nsp9

Deposition date:

2021-01-21

Original release date:

2021-02-22

Authors:

El-Kamand, Serene; Du Plessis, Mar-Dean; Cubeddu, Liza; Gamsjaeger, Roland

Citation:

Citation: El-Kamand, Serene; Du Plessis, Mar-Dean; Breen, Natasha; Johnson, Lexie; Beard, Samuel; Kwan, Ann; Richard, Derek; Cubeddu, Liza; Gamsjaeger, Roland. "A distinct ssDNA/RNA binding interface in the Nsp9 protein from SARS-CoV-2" Proteins 90, 176-185 (2022).
PubMed: 34369011

Assembly members:

Assembly members:
entity_1, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NNELSPVALRQMSCAAGTTQ TACTDDNALAYYNTTKGGRF VLALLSDLQDLKWARFPKSD GTGTIYTELEPPCRFVTDTP KGPKVKYLYFIKGLNNLNRG MVLGSLAATVRLQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	172
15N chemical shifts	80
1H chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nsp9	1

Entities:

Entity 1, Nsp9 113 residues - Formula weight is not available

1

ASN

GLU

LEU

SER

PRO

VAL

ALA

LEU

ARG

2

GLN

MET

SER

CYS

ALA

GLY

THR

GLN

3

THR

ALA

CYS

THR

ASP

ASN

ALA

LEU

ALA

4

TYR

ASN

THR

LYS

GLY

ARG

PHE

5

VAL

LEU

ALA

LEU

SER

ASP

LEU

GLN

ASP

6

LEU

LYS

TRP

ALA

ARG

PHE

PRO

LYS

SER

ASP

7

GLY

THR

GLY

THR

ILE

TYR

THR

GLU

LEU

GLU

8

PRO

CYS

ARG

PHE

VAL

THR

ASP

THR

PRO

9

LYS

GLY

PRO

LYS

VAL

LYS

TYR

LEU

TYR

PHE

10

ILE

LYS

GLY

LEU

ASN

LEU

ASN

ARG

GLY

11

MET

VAL

LEU

GLY

SER

LEU

ALA

THR

VAL

12

ARG

LEU

GLN

Samples:

sample_1: Nsp9, [U-100% 13C; U-100% 15N], 0.935 mM; sodium phosphate 25 mM; NaCl 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks