BMRB Entry 50719
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50719
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Citation: Weber, Daniel; Reddy, U Venkateswara; Wang, Songlin; Larsen, Erik; Gopinath, Tata; Gustavsson, Martin; Cornea, Razvan; Thomas, David; De Simone, Alfonso; Veglia, Gianluigi. "Structural basis for allosteric control of the SERCA-Phospholamban membrane complex by Ca 2+ and phosphorylation" Elife 10, e66226-e66226 (2021).
PubMed: 33978571
Assembly members:
entity_2, polymer, 53 residues, 6246 Da.
Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMal-c2x
Entity Sequences (FASTA):
entity_2: AMEKVQYLTRSAIRRASTIE
MPQQARQNLQNLFINFALIL
IFLLLIAIIVMLL
- assigned_chemical_shifts
- dipolar_couplings
| Data type | Count |
| 15N chemical shifts | 22 |
| dipolar coupling values | 22 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pPLN | 1 |
Entities:
Entity 1, pPLN 53 residues - 6246 Da.
Monomeric cysteine-null mutant (C36A/C41F/C46A). N-terminal Ala0 left over from cleavage of MBP fusion protein. Phosphorylated at Ser16 by protein kinase A.
| 1 | ALA | MET | GLU | LYS | VAL | GLN | TYR | LEU | THR | ARG | ||||
| 2 | SER | ALA | ILE | ARG | ARG | ALA | SEP | THR | ILE | GLU | ||||
| 3 | MET | PRO | GLN | GLN | ALA | ARG | GLN | ASN | LEU | GLN | ||||
| 4 | ASN | LEU | PHE | ILE | ASN | PHE | ALA | LEU | ILE | LEU | ||||
| 5 | ILE | PHE | LEU | LEU | LEU | ILE | ALA | ILE | ILE | VAL | ||||
| 6 | MET | LEU | LEU |
Samples:
sample_1: Ser16-phosphorylated phospholamban (pPLN-AFA), [U-15N], 3 mM; HEPES 20 mM; potassium chloride 100 mM; sodium azide 1 mM; glycerol 2.5%; 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) 340 mM; 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) 85 mM; 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) 106 mM; 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (PE-DTPA) 6.4 mM; Ytterbium(III) chloride 5 mM
sample_conditions_1: pH: 4.2; pressure: 1 atm; temperature: 303.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NH SLF | sample_1 | anisotropic | sample_conditions_1 |
Software:
NMRPipe - processing
VNMRj - collection
NMRFAM-SPARKY - data analysis
NMR spectrometers:
- Varian VXRS 700 MHz