BMRB Entry 50715

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50715

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Title:
Solution NMR backbone assignments of the N-terminal Za-linker-Zb segment from Homo Sapiens ADAR1p150.
Deposition date:
2021-01-16
Original release date:
2021-03-21
Authors:
Nichols, Parker; Henen, Morkos; Vicens, Quentin; Vogeli, Beat
Citation:

Citation: Nichols, Parker; Henen, Morkos; Vicens, Quentin; Vogeli, Beat. "Solution NMR backbone assignments of the N-terminal Zalpha-linker-Zbeta segment from Homo sapiens ADAR1p150"  Biomol. NMR Assign. 15, 273-279 (2021).
PubMed: 33742389

Assembly members:

Assembly members:
entity_1, polymer, 227 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet-28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEQRILKFLEELGEGKATTA HDLSGKLGTPKKEINRVLYS LAKKGKLQKEAGTPPLWKIA VSTQAWNQHSGVVRPDGHSQ GAPNSDPSLEPEDRNSSVSE DLLEPFIAVSAQAWNQHSGV VRPDSHSQGSPNSDPGLEPE DSNSTSALEDPLEFLDMAEI KEKICDYLFNVSDSSALNLA KNIGLTKARDINAVLIDMER QGDVYRQGTTPPIWHLTDKK RERMQIK

Data sets:
Data typeCount
13C chemical shifts643
15N chemical shifts211
1H chemical shifts211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Za-Zb1

Entities:

Entity 1, Za-Zb 227 residues - Formula weight is not available

1   METGLUGLNARGILELEULYSPHELEUGLU
2   GLULEUGLYGLUGLYLYSALATHRTHRALA
3   HISASPLEUSERGLYLYSLEUGLYTHRPRO
4   LYSLYSGLUILEASNARGVALLEUTYRSER
5   LEUALALYSLYSGLYLYSLEUGLNLYSGLU
6   ALAGLYTHRPROPROLEUTRPLYSILEALA
7   VALSERTHRGLNALATRPASNGLNHISSER
8   GLYVALVALARGPROASPGLYHISSERGLN
9   GLYALAPROASNSERASPPROSERLEUGLU
10   PROGLUASPARGASNSERSERVALSERGLU
11   ASPLEULEUGLUPROPHEILEALAVALSER
12   ALAGLNALATRPASNGLNHISSERGLYVAL
13   VALARGPROASPSERHISSERGLNGLYSER
14   PROASNSERASPPROGLYLEUGLUPROGLU
15   ASPSERASNSERTHRSERALALEUGLUASP
16   PROLEUGLUPHELEUASPMETALAGLUILE
17   LYSGLULYSILECYSASPTYRLEUPHEASN
18   VALSERASPSERSERALALEUASNLEUALA
19   LYSASNILEGLYLEUTHRLYSALAARGASP
20   ILEASNALAVALLEUILEASPMETGLUARG
21   GLNGLYASPVALTYRARGGLNGLYTHRTHR
22   PROPROILETRPHISLEUTHRASPLYSLYS
23   ARGGLUARGMETGLNILELYS

Samples:

sample_1: Za-Zb, [U-13C; U-15N; U-2H], 680 uM; potassium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D TROSY HNCACBsample_1isotropicsample_conditions_1
3D TROSY HN(CO)CACBsample_1isotropicsample_conditions_1
3D TROSY HN(CA)COsample_1isotropicsample_conditions_1
3D TROSY HNCOsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1

Software:

NMRDraw v10.9 - processing

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Bruker Ultrashield 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks