BMRB Entry 5071

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5071
MolProbity Validation Chart

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Title:
Complex of the C-Domain of Troponin C with Residues 1-40 of Troponin I
Deposition date:
2001-07-09
Original release date:
2001-11-14
Authors:
Mercier, P.; Spyracopoulos, L.; Sykes, B.
Citation:

Citation: Mercier, P.; Spyracopoulos, L.; Sykes, B.. "Structure, Dynamics, and Thermodynamics of the Structural Domain of Troponin C in Complex with the Regulatory Peptide 1-40 of Troponin I "  Biochemistry 40, 10063-10077 (2001).
PubMed: 11513585

Assembly members:

Assembly members:
TROPONIN C, polymer, 77 residues, Formula weight is not available
TROPONIN I, polymer, 40 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TROPONIN C: MMEDAKGKSEEELANCFRIF DKNADGFIDIEELGEILRAT GEHVIEEDIEDLMKDSDKNN DGRIDFDEFLKMMEGVQ
TROPONIN I: GDEEKRNRAITARRQHLKSV MLQIAATELEKEEGRREAEK

Data sets:
Data typeCount
1H chemical shifts451
13C chemical shifts228
15N chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TROPONIN C1
2TROPONIN I (1-40)2
3CALCIUM ION I3
4CALCIUM ION II3

Entities:

Entity 1, TROPONIN C 77 residues - Formula weight is not available

1   METMETGLUASPALALYSGLYLYSSERGLU
2   GLUGLULEUALAASNCYSPHEARGILEPHE
3   ASPLYSASNALAASPGLYPHEILEASPILE
4   GLUGLULEUGLYGLUILELEUARGALATHR
5   GLYGLUHISVALILEGLUGLUASPILEGLU
6   ASPLEUMETLYSASPSERASPLYSASNASN
7   ASPGLYARGILEASPPHEASPGLUPHELEU
8   LYSMETMETGLUGLYVALGLN

Entity 2, TROPONIN I (1-40) 40 residues - Formula weight is not available

1   GLYASPGLUGLULYSARGASNARGALAILE
2   THRALAARGARGGLNHISLEULYSSERVAL
3   METLEUGLNILEALAALATHRGLULEUGLU
4   LYSGLUGLUGLYARGARGGLUALAGLULYS

Entity 3, CALCIUM ION I - Ca - 40.078 Da.

1   CA

Samples:

sample_1: TROPONIN C, [U-15N; U-13C], 1.3 mM; TROPONIN I 1.9 mM; KCl 100 mM; Imidazole 10 mM; DSS 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.8; temperature: 303 K; ionic strength: 0.1 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablesample_cond_1
3D 13C-separated NOESYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1
HNHBsample_1not availablesample_cond_1
HNCACBsample_1not availablesample_cond_1
CBCACONNHsample_1not availablesample_cond_1
DIPSI-HSQCsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1

Software:

X-PLOR v3.1 - refinement

NMRPipe v1.8 Rev 2000.081.21.00 - processing

NMRview v4.0.3 - data analysis

PIPP v4.2 - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 500 MHz
  • Varian UNITY 600 MHz

Related Database Links:

PDB
EMBL CAA24262 CAA32392 CAB59981 CAM23286 CAM23287 CAA32392 CAM23285 CAA24262
GB AAA19813 AAA31490 AAA40485 AAA42149 AAB26824
PRF 1914142A 1914142A
REF NP_001076252 NP_001139301 NP_001139313 NP_001179023 NP_003273 NP_058881 NP_001076252 NP_033431
SP P02643 P13412 P27768 P48788
TPG DAA13498
SWISS-PROT P13412 P27768 P02643
GenBank AAH28515 AAA42149 AAB26824 AAA31490 AAA40485
AlphaFold P02643 P13412 P27768 P48788

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