BMRB Entry 50702

Name: Degron-tagged Ig2D5
Published: 2021-05-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50702

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Degron-tagged Ig2D5

Deposition date:

2021-01-08

Original release date:

2021-05-18

Authors:

Tzeng, Shiou-Ru

Citation:

Citation: Tzeng, Shiou-Ru; Tseng, Yin-Chu; Lin, Chien-Chu; Hsu, Chia-Ying; Huang, Shing-Jong; Kuo, Yi-Ting; Chang, Chung-I. "Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease" elife 10, e64056-e64056 (2021).
PubMed: 33929321

Assembly members:

Assembly members:
entity_1, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Dictyostelium discoideum Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET_Ig2D5_S20

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMTVKPAPSAEHSYAEGEG LVKVFDNAPAEFTIFAVDTK GVARTDGGDPFEVAINGPDG LVVDAKVTDNNDGTYGVVYD APVEGNYNVNVTLRGNPIKN MPIDVKCIEGASSHATRQLS GLKIHSNLYH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	215
15N chemical shifts	115
1H chemical shifts	115

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Degron tag Sul20	1

Entities:

Entity 1, Degron tag Sul20 130 residues - Formula weight is not available

A degron-tagged Ig2D5 is a chimeric protein: Sul20 (the C-terminal 20 residues of SulA; namely Sul20) is appended to the C terminus of a protein substrate Ig2D5 (domains 5 of the gelation factor from Dictyostelium discoideum. The sequence of Ig2D5: GHMTVKPAPSAEHSYAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDGGDPFEVAINGPDGLVVDAKVTDNNDGTYGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKCIEG The sequence of Sul20: ASSHATRQLSGLKIHSNLYH

1	GLY	HIS	MET	THR	VAL	LYS	PRO	ALA	PRO	SER
2	ALA	GLU	HIS	SER	TYR	ALA	GLU	GLY	GLU	GLY
3	LEU	VAL	LYS	VAL	PHE	ASP	ASN	ALA	PRO	ALA
4	GLU	PHE	THR	ILE	PHE	ALA	VAL	ASP	THR	LYS
5	GLY	VAL	ALA	ARG	THR	ASP	GLY	GLY	ASP	PRO
6	PHE	GLU	VAL	ALA	ILE	ASN	GLY	PRO	ASP	GLY
7	LEU	VAL	VAL	ASP	ALA	LYS	VAL	THR	ASP	ASN
8	ASN	ASP	GLY	THR	TYR	GLY	VAL	VAL	TYR	ASP
9	ALA	PRO	VAL	GLU	GLY	ASN	TYR	ASN	VAL	ASN
10	VAL	THR	LEU	ARG	GLY	ASN	PRO	ILE	LYS	ASN
11	MET	PRO	ILE	ASP	VAL	LYS	CYS	ILE	GLU	GLY
12	ALA	SER	SER	HIS	ALA	THR	ARG	GLN	LEU	SER
13	GLY	LEU	LYS	ILE	HIS	SER	ASN	LEU	TYR	HIS

Samples:

sample_1: A degron-tagged Ig2D5, [U-100% 13C; U-100% 15N], 0.3 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVIII-800 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	GLY	HIS	MET	THR	VAL	LYS	PRO	ALA	PRO	SER
2	ALA	GLU	HIS	SER	TYR	ALA	GLU	GLY	GLU	GLY
3	LEU	VAL	LYS	VAL	PHE	ASP	ASN	ALA	PRO	ALA
4	GLU	PHE	THR	ILE	PHE	ALA	VAL	ASP	THR	LYS
5	GLY	VAL	ALA	ARG	THR	ASP	GLY	GLY	ASP	PRO
6	PHE	GLU	VAL	ALA	ILE	ASN	GLY	PRO	ASP	GLY
7	LEU	VAL	VAL	ASP	ALA	LYS	VAL	THR	ASP	ASN
8	ASN	ASP	GLY	THR	TYR	GLY	VAL	VAL	TYR	ASP
9	ALA	PRO	VAL	GLU	GLY	ASN	TYR	ASN	VAL	ASN
10	VAL	THR	LEU	ARG	GLY	ASN	PRO	ILE	LYS	ASN
11	MET	PRO	ILE	ASP	VAL	LYS	CYS	ILE	GLU	GLY
12	ALA	SER	SER	HIS	ALA	THR	ARG	GLN	LEU	SER
13	GLY	LEU	LYS	ILE	HIS	SER	ASN	LEU	TYR	HIS

1	GLY	HIS	MET	THR	VAL	LYS	PRO	ALA	PRO	SER
2	ALA	GLU	HIS	SER	TYR	ALA	GLU	GLY	GLU	GLY
3	LEU	VAL	LYS	VAL	PHE	ASP	ASN	ALA	PRO	ALA
4	GLU	PHE	THR	ILE	PHE	ALA	VAL	ASP	THR	LYS
5	GLY	VAL	ALA	ARG	THR	ASP	GLY	GLY	ASP	PRO
6	PHE	GLU	VAL	ALA	ILE	ASN	GLY	PRO	ASP	GLY
7	LEU	VAL	VAL	ASP	ALA	LYS	VAL	THR	ASP	ASN
8	ASN	ASP	GLY	THR	TYR	GLY	VAL	VAL	TYR	ASP
9	ALA	PRO	VAL	GLU	GLY	ASN	TYR	ASN	VAL	ASN
10	VAL	THR	LEU	ARG	GLY	ASN	PRO	ILE	LYS	ASN
11	MET	PRO	ILE	ASP	VAL	LYS	CYS	ILE	GLU	GLY
12	ALA	SER	SER	HIS	ALA	THR	ARG	GLN	LEU	SER
13	GLY	LEU	LYS	ILE	HIS	SER	ASN	LEU	TYR	HIS

1	GLY	HIS	MET	THR	VAL	LYS	PRO	ALA	PRO	SER
2	ALA	GLU	HIS	SER	TYR	ALA	GLU	GLY	GLU	GLY
3	LEU	VAL	LYS	VAL	PHE	ASP	ASN	ALA	PRO	ALA
4	GLU	PHE	THR	ILE	PHE	ALA	VAL	ASP	THR	LYS
5	GLY	VAL	ALA	ARG	THR	ASP	GLY	GLY	ASP	PRO
6	PHE	GLU	VAL	ALA	ILE	ASN	GLY	PRO	ASP	GLY
7	LEU	VAL	VAL	ASP	ALA	LYS	VAL	THR	ASP	ASN
8	ASN	ASP	GLY	THR	TYR	GLY	VAL	VAL	TYR	ASP
9	ALA	PRO	VAL	GLU	GLY	ASN	TYR	ASN	VAL	ASN
10	VAL	THR	LEU	ARG	GLY	ASN	PRO	ILE	LYS	ASN
11	MET	PRO	ILE	ASP	VAL	LYS	CYS	ILE	GLU	GLY
12	ALA	SER	SER	HIS	ALA	THR	ARG	GLN	LEU	SER
13	GLY	LEU	LYS	ILE	HIS	SER	ASN	LEU	TYR	HIS