BMRB Entry 50701

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50701

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Title:
htau40 assignment
Deposition date:
2021-01-07
Original release date:
2021-02-11
Authors:
Zweckstetter, Anna Siegert
Citation:

Citation: Narayanan, Rhagavendran; Durr, Ulrich; Bibow, Stefan; Biernat, Jacek; Mandelkow, Eckhard; Zweckstetter, Markus. "Automatic assignment of the intrinsically disordered protein Tau with 441-residues."  J. Am. Chem. Soc. 132, 11906-11907 (2010).
PubMed: 20687558

Assembly members:

Assembly members:
entity_1, polymer, 441 residues, 45849.91 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNG2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAEPRQEFEVMEDHAGTYGL GDRKDQGGYTMHQDQEGDTD AGLKESPLQTPTEDGSEEPG SETSDAKSTPTAEDVTAPLV DEGAPGKQAAAQPHTEIPEG TTAEEAGIGDTPSLEDEAAG HVTQARMVSKSKDGTGSDDK KAKGADGKTKIATPRGAAPP GQKGQANATRIPAKTPPAPK TPPSSGEPPKSGDRSGYSSP GSPGTPGSRSRTPSLPTPPT REPKKVAVVRTPPKSPSSAK SRLQTAPVPMPDLKNVKSKI GSTENLKHQPGGGKVQIINK KLDLSNVQSKCGSKDNIKHV PGGGSVQIVYKPVDLSKVTS KCGSLGNIHHKPGGGQVEVK SEKLDFKDRVQSKIGSLDNI THVPGGGNKKIETHKLTFRE NAKAKTDHGAEIVYKSPVVS GDTSPRHLSNVSSTGSIDMV DSPQLATLADEVSASLAKQG L

Data sets:
Data typeCount
15N chemical shifts362
1H chemical shifts362

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1htau401

Entities:

Entity 1, htau40 441 residues - 45849.91 Da.

1   METALAGLUPROARGGLNGLUPHEGLUVAL
2   METGLUASPHISALAGLYTHRTYRGLYLEU
3   GLYASPARGLYSASPGLNGLYGLYTYRTHR
4   METHISGLNASPGLNGLUGLYASPTHRASP
5   ALAGLYLEULYSGLUSERPROLEUGLNTHR
6   PROTHRGLUASPGLYSERGLUGLUPROGLY
7   SERGLUTHRSERASPALALYSSERTHRPRO
8   THRALAGLUASPVALTHRALAPROLEUVAL
9   ASPGLUGLYALAPROGLYLYSGLNALAALA
10   ALAGLNPROHISTHRGLUILEPROGLUGLY
11   THRTHRALAGLUGLUALAGLYILEGLYASP
12   THRPROSERLEUGLUASPGLUALAALAGLY
13   HISVALTHRGLNALAARGMETVALSERLYS
14   SERLYSASPGLYTHRGLYSERASPASPLYS
15   LYSALALYSGLYALAASPGLYLYSTHRLYS
16   ILEALATHRPROARGGLYALAALAPROPRO
17   GLYGLNLYSGLYGLNALAASNALATHRARG
18   ILEPROALALYSTHRPROPROALAPROLYS
19   THRPROPROSERSERGLYGLUPROPROLYS
20   SERGLYASPARGSERGLYTYRSERSERPRO
21   GLYSERPROGLYTHRPROGLYSERARGSER
22   ARGTHRPROSERLEUPROTHRPROPROTHR
23   ARGGLUPROLYSLYSVALALAVALVALARG
24   THRPROPROLYSSERPROSERSERALALYS
25   SERARGLEUGLNTHRALAPROVALPROMET
26   PROASPLEULYSASNVALLYSSERLYSILE
27   GLYSERTHRGLUASNLEULYSHISGLNPRO
28   GLYGLYGLYLYSVALGLNILEILEASNLYS
29   LYSLEUASPLEUSERASNVALGLNSERLYS
30   CYSGLYSERLYSASPASNILELYSHISVAL
31   PROGLYGLYGLYSERVALGLNILEVALTYR
32   LYSPROVALASPLEUSERLYSVALTHRSER
33   LYSCYSGLYSERLEUGLYASNILEHISHIS
34   LYSPROGLYGLYGLYGLNVALGLUVALLYS
35   SERGLULYSLEUASPPHELYSASPARGVAL
36   GLNSERLYSILEGLYSERLEUASPASNILE
37   THRHISVALPROGLYGLYGLYASNLYSLYS
38   ILEGLUTHRHISLYSLEUTHRPHEARGGLU
39   ASNALALYSALALYSTHRASPHISGLYALA
40   GLUILEVALTYRLYSSERPROVALVALSER
41   GLYASPTHRSERPROARGHISLEUSERASN
42   VALSERSERTHRGLYSERILEASPMETVAL
43   ASPSERPROGLNLEUALATHRLEUALAASP
44   GLUVALSERALASERLEUALALYSGLNGLY
45   LEU

Samples:

sample_1: Microtubule-associated protein tau, [U-100% 13C; U-100% 15N], 1.3 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
APSYsample_1isotropicsample_conditions_1

Software:

NMRPipe v9.9 - chemical shift assignment, chemical shift calculation

MARS - chemical shift assignment

PROSA - data analysis

NMR spectrometers:

  • Bruker AVANCE III 900 MHz
  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks