BMRB Entry 50699
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50699
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Bartholow, Thomas; Sztain, Terra; Patel, Ashay; Lee, D John; Young, Megan; Abagyan, Ruben; Burkart, Michael. "Elucidation of transient protein-protein interactions within carrier protein-dependent biosynthesis" Commun. Biol. 4, 340-340 (2021).
PubMed: 33727677
Assembly members:
entity_1, polymer, 74 residues, Formula weight is not available
entity_2, polymer, 172 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet28b
Entity Sequences (FASTA):
entity_1: TIEERVKKIIGEQLGVKQEE
VTNNASFVEDLGADSLDTVE
LVMALEEEFDTEIPDEEAEK
ITTVQAAIDYINGH
entity_2: MVDKRESYTKEDLLASGRGE
LFGAKGPQLPAPNMLMMDRV
VKMTETGGNFDKGYVEAELD
INPDLWFFGCHFIGDPVMPG
CLGLDAMWQLVGFYLGWLGG
EGKGRALGVGEVKFTGQVLP
TAKKVTYRIHFKRIVNRRLI
MGLADGEVLVDGRLIYTASD
LKVGLFQDTSAF
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AcpP | 1 |
| 2 | FabA | 2 |
Entities:
Entity 1, AcpP 74 residues - Formula weight is not available
| 1 | THR | ILE | GLU | GLU | ARG | VAL | LYS | LYS | ILE | ILE | ||||
| 2 | GLY | GLU | GLN | LEU | GLY | VAL | LYS | GLN | GLU | GLU | ||||
| 3 | VAL | THR | ASN | ASN | ALA | SER | PHE | VAL | GLU | ASP | ||||
| 4 | LEU | GLY | ALA | ASP | SER | LEU | ASP | THR | VAL | GLU | ||||
| 5 | LEU | VAL | MET | ALA | LEU | GLU | GLU | GLU | PHE | ASP | ||||
| 6 | THR | GLU | ILE | PRO | ASP | GLU | GLU | ALA | GLU | LYS | ||||
| 7 | ILE | THR | THR | VAL | GLN | ALA | ALA | ILE | ASP | TYR | ||||
| 8 | ILE | ASN | GLY | HIS |
Entity 2, FabA 172 residues - Formula weight is not available
| 1 | MET | VAL | ASP | LYS | ARG | GLU | SER | TYR | THR | LYS | ||||
| 2 | GLU | ASP | LEU | LEU | ALA | SER | GLY | ARG | GLY | GLU | ||||
| 3 | LEU | PHE | GLY | ALA | LYS | GLY | PRO | GLN | LEU | PRO | ||||
| 4 | ALA | PRO | ASN | MET | LEU | MET | MET | ASP | ARG | VAL | ||||
| 5 | VAL | LYS | MET | THR | GLU | THR | GLY | GLY | ASN | PHE | ||||
| 6 | ASP | LYS | GLY | TYR | VAL | GLU | ALA | GLU | LEU | ASP | ||||
| 7 | ILE | ASN | PRO | ASP | LEU | TRP | PHE | PHE | GLY | CYS | ||||
| 8 | HIS | PHE | ILE | GLY | ASP | PRO | VAL | MET | PRO | GLY | ||||
| 9 | CYS | LEU | GLY | LEU | ASP | ALA | MET | TRP | GLN | LEU | ||||
| 10 | VAL | GLY | PHE | TYR | LEU | GLY | TRP | LEU | GLY | GLY | ||||
| 11 | GLU | GLY | LYS | GLY | ARG | ALA | LEU | GLY | VAL | GLY | ||||
| 12 | GLU | VAL | LYS | PHE | THR | GLY | GLN | VAL | LEU | PRO | ||||
| 13 | THR | ALA | LYS | LYS | VAL | THR | TYR | ARG | ILE | HIS | ||||
| 14 | PHE | LYS | ARG | ILE | VAL | ASN | ARG | ARG | LEU | ILE | ||||
| 15 | MET | GLY | LEU | ALA | ASP | GLY | GLU | VAL | LEU | VAL | ||||
| 16 | ASP | GLY | ARG | LEU | ILE | TYR | THR | ALA | SER | ASP | ||||
| 17 | LEU | LYS | VAL | GLY | LEU | PHE | GLN | ASP | THR | SER | ||||
| 18 | ALA | PHE |
Samples:
sample_1: E. coli Acyl Carrier Protein, [U-100% 15N; U~90% 2H], 0.220 uM; E. coli 3-oxoacyl-[acyl-carrier-protein] synthase 2 1.226 mM
sample_2: E. coli Acyl Carrier Protein, [U-100% 15N; U~90% 2H], 0.220 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC AcpP FabA titration saturated | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC AcpP FabA titration zero point | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMR Pipe - processing
SPARKY - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks