BMRB Entry 50687

Name: SARS CoV-2 Non structural protein 2 C-terminal instrinsically disordered region (Nsp2 CtIDR)
Published: 2021-11-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50687

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SARS CoV-2 Non structural protein 2 C-terminal instrinsically disordered region (Nsp2 CtIDR)

Deposition date:

2020-12-30

Original release date:

2021-11-14

Authors:

Trevino, Miguel; Mompean, Miguel; Laurents, Douglas

Citation:

Citation: Mompean, Miguel; Trevino, Miguel; Laurents, Douglas. "Partial structure, dampened mobility, and modest impact of a His tag in the SARS-CoV-2 Nsp2 C-terminal region" Eur. Biophys. J. 50, 1129-1137 (2021).
PubMed: 34633480

Assembly members:

Assembly members:
entity_1, polymer, 45 residues, 4865 Da.

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KEIIFLEGETLPTEVLTEEV VLKTGDLQPLEQPTSEAVEA PLVGT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	125
15N chemical shifts	42
1H chemical shifts	40

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SARS CoV-2 Nsp2, C-terminal Intrinsically Disordered Region (Residues 557-601)	1

Entities:

Entity 1, SARS CoV-2 Nsp2, C-terminal Intrinsically Disordered Region (Residues 557-601) 45 residues - 4865 Da.

1

LYS

GLU

ILE

PHE

LEU

GLU

GLY

GLU

THR

2

LEU

PRO

THR

GLU

VAL

LEU

THR

GLU

VAL

3

VAL

LEU

LYS

THR

GLY

ASP

LEU

GLN

PRO

LEU

4

GLU

GLN

PRO

THR

SER

GLU

ALA

VAL

GLU

ALA

5

PRO

LEU

VAL

GLY

THR

Samples:

sample_1: SARS CoV-2 Nsp2 (Residues 557-601), [U-98% 13C; U-98% 15N], 1.35 mM; NaCl 10 mM; NaH2PO4 5 mM

sample_conditions_1: ionic strength: 0.03 M; pH: 6.3; pressure: 0.92 atm; temperature: 278.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_1	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
2D CON	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.13 - peak picking, assist manual assignment

TOPSPIN v4.0.8 - Collection, transformation and processing of spectra

NMR spectrometers:

Bruker AVANCE NEO 800 MHz