BMRB Entry 50680

Name: Red1 backbone chemical shift assignments for residues 288-322
Published: 2022-11-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50680

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Red1 backbone chemical shift assignments for residues 288-322

Deposition date:

2020-12-22

Original release date:

2022-11-30

Authors:

Soni, Komal; Sinning, Irmgard

Citation:

Citation: Soni, Komal; Sivadas, Anusree; Horvath, Attila; Dobrev, Nikolay; Hayashi, Rippei; Kiss, Leo; Simon, Bernd; Wild, Klemens; Sinning, Irmgard; Fischer, Tamas. "Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex" Nat. Commun. 14, 772-772 (2023).
PubMed: 36774373

Assembly members:

Assembly members:
entity_1, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fission yeast Taxonomy ID: 1893 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKQYKLILNGKTLKGETTTE AVDAATAEKVFKQYANDNGV DGEWTYDDATKTFTVTEGSG SGSENLYFQGAMGISLPLLK QDDWLSSSKPFGSSTPNVVI EFDSDDDGSHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	305
15N chemical shifts	106
1H chemical shifts	105

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GB1-TEV-Red1	1

Entities:

Entity 1, GB1-TEV-Red1 115 residues - Formula weight is not available

The sequence contains GB1-tag and TEV protease cleavage site at the N-terminus followed by Red1 residues 288-322 and a C-terminal 6xHis-tag.

1

MET

LYS

GLN

TYR

LYS

LEU

ILE

LEU

ASN

GLY

2

LYS

THR

LEU

LYS

GLY

GLU

THR

GLU

3

ALA

VAL

ASP

ALA

THR

ALA

GLU

LYS

VAL

4

PHE

LYS

GLN

TYR

ALA

ASN

ASP

ASN

GLY

VAL

5

ASP

GLY

GLU

TRP

THR

TYR

ASP

ALA

THR

6

LYS

THR

PHE

THR

VAL

THR

GLU

GLY

SER

GLY

7

SER

GLY

SER

GLU

ASN

LEU

TYR

PHE

GLN

GLY

8

ALA

MET

GLY

ILE

SER

LEU

PRO

LEU

LYS

9

GLN

ASP

TRP

LEU

SER

LYS

PRO

10

PHE

GLY

SER

THR

PRO

ASN

VAL

ILE

11

GLU

PHE

ASP

SER

ASP

GLY

SER

HIS

12

HIS

Samples:

sample_1: GB1-TEV-Red1, [U-100% 13C; U-100% 15N], 1 mM; Sodium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

ANALYSIS - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks