BMRB Entry 50674

Title:
3_UTR
Deposition date:
2020-12-20
Original release date:
2022-02-01
Authors:
Schwalbe, Harald; Sreeramulu, Sridhar; Richter, Christian
Citation:

Citation: Sreeramulu, Sridhar; Richter, Christian; Berg, Hannes; Wirtz Martin, Maria; Ceylan, Betul; Matzel, Tobias; Adam, Jennifer; Altincekic, Nadide; Azzaoui, Kamal; Bains, Jasleen Kaur; Blommers, Marcel; Ferner, Jan; Furtig, Boris; Gobel, M.; Grun, J Tassilo; Hengesbach, Martin; Hohmann, Katharina; Hymon, Daniel; Knezic, Bozana; Martins, Jason; Mertinkus, Klara; Niesteruk, Anna; Peter, Stephen; Pyper, Dennis; Qureshi, Nusrat; Scheffer, Ute; Schlundt, Andreas; Schnieders, Robbin; Stirnal, Elke; Sudakov, Alexey; Troster, Alix; Vogele, Jennifer; Wacker, Anna; Weigand, Julia; Wirmer-Bartoschek, Julia; Wohnert, Jens; Schwalbe, Harald. "Exploring the druggability of conserved RNA regulatory elements in the SARS-CoV-2 genome"  Angew. Chem. Int. Ed. Engl. 60, 19191-19200 (2021).
PubMed: 34161644

Assembly members:

Assembly members:
entity_1, polymer, 337 residues, Formula weight is not available
entity_2, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: transcription   Host organism: in-vitro

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
13_UTR1
2DSI-Poised Library (DSI-PL)2

Entities:

Entity 1, 3_UTR 337 residues - Formula weight is not available

1   ACUCAUGCAG
2   ACCACACAAG
3   GCAGAUGGGC
4   UAUAUAAACG
5   UUUUCGCUUU
6   UCCGUUUACG
7   AUAUAUAGUC
8   UACUCUUGUG
9   CAGAAUGAAU
10   UCUCGUAACU
11   ACAUAGCACA
12   AGUAGAUGUA
13   GUUAACUUUA
14   AUCUCACAUA
15   GCAAUCUUUA
16   AUCAGUGUGU
17   AACAUUAGGG
18   AGGACUUGAA
19   AGAGCCACCA
20   CAUUUUCACC
21   GAGGCCACGC
22   GGAGUACGAU
23   CGAGUGUACA
24   GUGAACAAUG
25   CUAGGGAGAG
26   CUGCCUAUAU
27   GGAAGAGCCC
28   UAAUGUGUAA
29   AAUUAAUUUU
30   AGUAGUGCUA
31   UCCCCAUGUG
32   AUUUUAAUAG
33   CUUCUUAGGA
34   GAAUGAC

Entity 2, DSI-Poised Library (DSI-PL) - Formula weight is not available

Samples:

sample_1: 3_UTR 10 uM; potassium phosphate 25 mM; KCl 50 mM; H2O 95%; DMSO, [U-100% 2H], 5%; Fragment 1 200 uM; Fragment 2 200 uM; Fragment 3 200 uM; Fragment 4 200 uM; Fragment 5 200 uM; Fragment 6 200 uM; Fragment 7 200 uM; Fragment 8 200 uM; Fragment 9 200 uM; Fragment 10 200 uM; Fragment 11 200 uM; Fragment 12 200 uM

sample_2: potassium phosphate 25 mM; KCl 50 mM; H2O 95%; DMSO, [U-100% 2H], 5%; Fragment 1 200 uM; Fragment 2 200 uM; Fragment 3 200 uM; Fragment 4 200 uM; Fragment 5 200 uM; Fragment 6 200 uM; Fragment 7 200 uM; Fragment 8 200 uM; Fragment 9 200 uM; Fragment 10 200 uM; Fragment 11 200 uM; Fragment 12 200 uM

sample_conditions_1: ionic strength: 75 mM; pH: 6.2; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
1D 1H waterLOGSYsample_1isotropicsample_conditions_1
1H R2 CPMG 5mssample_1isotropicsample_conditions_1
1H R2 CPMG 100mssample_1isotropicsample_conditions_1
1D 1H refsample_2isotropicsample_conditions_1
1D 1H waterLOGSY refsample_2isotropicsample_conditions_1
1H R2 CPMG 5ms refsample_2isotropicsample_conditions_1
1H R2 CPMG 100ms refsample_2isotropicsample_conditions_1

Software:

LOGS v2.2 - collection

TOPSPIN v4.0.9 - collection, data analysis

NMR spectrometers:

  • Bruker Bruker Avance Neo 600 MHz