BMRB Entry 50673

Title:
5_UTR
Deposition date:
2020-12-20
Original release date:
2022-02-01
Authors:
Schwalbe, Harald; Sreeramulu, Sridhar; Richter, Christian
Citation:

Citation: Sreeramulu, Sridhar; Richter, Christian; Berg, Hannes; Wirtz Martin, Maria; Ceylan, Betul; Matzel, Tobias; Adam, Jennifer; Altincekic, Nadide; Azzaoui, Kamal; Bains, Jasleen Kaur; Blommers, Marcel; Ferner, Jan; Furtig, Boris; Gobel, M.; Grun, J Tassilo; Hengesbach, Martin; Hohmann, Katharina; Hymon, Daniel; Knezic, Bozana; Martins, Jason; Mertinkus, Klara; Niesteruk, Anna; Peter, Stephen; Pyper, Dennis; Qureshi, Nusrat; Scheffer, Ute; Schlundt, Andreas; Schnieders, Robbin; Stirnal, Elke; Sudakov, Alexey; Troster, Alix; Vogele, Jennifer; Wacker, Anna; Weigand, Julia; Wirmer-Bartoschek, Julia; Wohnert, Jens; Schwalbe, Harald. "Exploring the druggability of conserved RNA regulatory elements in the SARS-CoV-2 genome"  Angew. Chem. Int. Ed. Engl. 60, 19191-19200 (2021).
PubMed: 34161644

Assembly members:

Assembly members:
entity_1, polymer, 472 residues, Formula weight is not available
entity_2, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: transcription   Host organism: in-vitro

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15_UTR1
2DSI-Poised Library (DSI-PL)2

Entities:

Entity 1, 5_UTR 472 residues - Formula weight is not available

1   AUUAAAGGUU
2   UAUACCUUCC
3   CAGGUAACAA
4   ACCAACCAAC
5   UUUCGAUCUC
6   UUGUAGAUCU
7   GUUCUCUAAA
8   CGAACUUUAA
9   AAUCUGUGUG
10   GCUGUCACUC
11   GGCUGCAUGC
12   UUAGUGCACU
13   CACGCAGUAU
14   AAUUAAUAAC
15   UAAUUACUGU
16   CGUUGACAGG
17   ACACGAGUAA
18   CUCGUCUAUC
19   UUCUGCAGGC
20   UGCUUACGGU
21   UUCGUCCGUG
22   UUGCAGCCGA
23   UCAUCAGCAC
24   AUCUAGGUUU
25   CGUCCGGGUG
26   UGACCGAAAG
27   GUAAGAUGGA
28   GAGCCUUGUC
29   CCUGGUUUCA
30   ACGAGAAAAC
31   ACACGUCCAA
32   CUCAGUUUGC
33   CUGUUUUACA
34   GGUUCGCGAC
35   GUGCUCGUAC
36   GUGGCUUUGG
37   AGACUCCGUG
38   GAGGAGGUCU
39   UAUCAGAGGC
40   ACGUCAACAU
41   CUUAAAGAUG
42   GCACUUGUGG
43   CUUAGUAGAA
44   GUUGAAAAAG
45   GCGUUUUGCC
46   UCAACUUGAA
47   CAGCCCUAUG
48   UG

Entity 2, DSI-Poised Library (DSI-PL) - Formula weight is not available

Samples:

sample_1: 5_UTR 10 uM; potassium phosphate 25 mM; KCl 50 mM; H2O 95%; DMSO, [U-100% 2H], 5%; Fragment 1 200 uM; Fragment 2 200 uM; Fragment 3 200 uM; Fragment 4 200 uM; Fragment 5 200 uM; Fragment 6 200 uM; Fragment 7 200 uM; Fragment 8 200 uM; Fragment 9 200 uM; Fragment 10 200 uM; Fragment 11 200 uM; Fragment 12 200 uM

sample_2: potassium phosphate 25 mM; KCl 50 mM; H2O 95%; DMSO, [U-100% 2H], 5%; Fragment 1 200 uM; Fragment 2 200 uM; Fragment 3 200 uM; Fragment 4 200 uM; Fragment 5 200 uM; Fragment 6 200 uM; Fragment 7 200 uM; Fragment 8 200 uM; Fragment 9 200 uM; Fragment 10 200 uM; Fragment 11 200 uM; Fragment 12 200 uM

sample_conditions_1: ionic strength: 75 mM; pH: 6.2; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
1D 1H waterLOGSYsample_1isotropicsample_conditions_1
1H R2 CPMG 5mssample_1isotropicsample_conditions_1
1H R2 CPMG 100mssample_1isotropicsample_conditions_1
1D 1H refsample_2isotropicsample_conditions_1
1D 1H waterLOGSY refsample_2isotropicsample_conditions_1
1H R2 CPMG 5ms refsample_2isotropicsample_conditions_1
1H R2 CPMG 100ms refsample_2isotropicsample_conditions_1

Software:

LOGS v2.2 - collection

TOPSPIN v4.0.9 - collection, data analysis

NMR spectrometers:

  • Bruker Bruker Avance III 600 MHz