BMRB Entry 5067
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5067
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Citation: Tozawa, Kaeko; Broadhurst, Richard; Raine, Andrew; Fuller, Christopher; Alvarez, Anabel; Guillen, Gerardo; Padron, Gabriel; Perham, Richard. "Solution Structure of the Lipoyl Domain of the Chimeric Dihydrolipoyl
Dehydrogenase P64K from Neisseria meningitidis" Eur. J. Biochem. 268, 4908-4917 (2001).
PubMed: 11559360
Assembly members:
lipoyl domain, polymer, 81 residues, 8323 Da.
Natural source: Common Name: Neisseria meningitidis Taxonomy ID: 487 Superkingdom: Eubacteria Kingdom: not available Genus/species: Neisseria meningitidis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
lipoyl domain: ALVELKVPDIGGHENVDIIA
VEVNVGDTIAVDDTLITLET
DKATMDVPAEVAGVVKEVKV
KVGDKISEGGLIVVVEAEGT
A
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 575 |
| 13C chemical shifts | 265 |
| 15N chemical shifts | 80 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p64K lipoyl domain | 1 |
Entities:
Entity 1, p64K lipoyl domain 81 residues - 8323 Da.
| 1 | ALA | LEU | VAL | GLU | LEU | LYS | VAL | PRO | ASP | ILE | ||||
| 2 | GLY | GLY | HIS | GLU | ASN | VAL | ASP | ILE | ILE | ALA | ||||
| 3 | VAL | GLU | VAL | ASN | VAL | GLY | ASP | THR | ILE | ALA | ||||
| 4 | VAL | ASP | ASP | THR | LEU | ILE | THR | LEU | GLU | THR | ||||
| 5 | ASP | LYS | ALA | THR | MET | ASP | VAL | PRO | ALA | GLU | ||||
| 6 | VAL | ALA | GLY | VAL | VAL | LYS | GLU | VAL | LYS | VAL | ||||
| 7 | LYS | VAL | GLY | ASP | LYS | ILE | SER | GLU | GLY | GLY | ||||
| 8 | LEU | ILE | VAL | VAL | VAL | GLU | ALA | GLU | GLY | THR | ||||
| 9 | ALA |
Samples:
sample_1: lipoyl domain, [U-2H; U-13C; U-15N], 2 mM
condition_1: pH*: 5.5; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| 1H-13C TOCSY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
Software:
ansig v3.3 - assignment
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| EMBL | CAA54878 CAA57206 CAA59171 CAA61894 CAA61895 |
| GB | AAF41719 AAW89295 ABX73429 ACF30036 ADO31781 |
| REF | NP_274363 WP_002213261 WP_002217021 WP_002219145 WP_002220847 |
Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone
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