BMRB Entry 5066

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5066
MolProbity Validation Chart

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Title:
1H and 15N Chemical Shift Assignments for the Alpha-domain of Mouse Metallothionein-3
Deposition date:
2001-07-01
Original release date:
2001-10-17
Authors:
Oz, Gulin; Zangger, Klaus; Armitage, Ian
Citation:

Citation: Oz, Gulin; Zangger, Klaus; Armitage, Ian. "Three-dimensional Structure and Dynamics of a Brain Specific Growth Inhibitory Factor: Metallothionein-3"  Biochemistry 40, 11433-11441 (2001).
PubMed: 11560491

Assembly members:

Assembly members:
Metallothionein-3, polymer, 68 residues, 7009 Da.
CD, non-polymer, 112.411 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Metallothionein-3: MDPETCPCPTGGSCTCSDKC KCKGCKCTNCKKSCCSCCPA GCEKCAKDCVCKGEEGAKAE AEKCSCCQ

Data sets:
Data typeCount
1H chemical shifts205
15N chemical shifts35

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1metallothionein-31
2Cadmium (II) ion, I2
3Cadmium (II) ion, II2
4Cadmium (II) ion, III2
5Cadmium (II) ion, IV2
6Cadmium (II) ion, V2
7Cadmium (II) ion, VI2
8Cadmium (II) ion, VII2

Entities:

Entity 1, metallothionein-3 68 residues - 7009 Da.

1   METASPPROGLUTHRCYSPROCYSPROTHR
2   GLYGLYSERCYSTHRCYSSERASPLYSCYS
3   LYSCYSLYSGLYCYSLYSCYSTHRASNCYS
4   LYSLYSSERCYSCYSSERCYSCYSPROALA
5   GLYCYSGLULYSCYSALALYSASPCYSVAL
6   CYSLYSGLYGLUGLUGLYALALYSALAGLU
7   ALAGLULYSCYSSERCYSCYSGLN

Entity 2, Cadmium (II) ion, I - Cd - 112.411 Da.

1   CD

Samples:

sample_1: Metallothionein-3, [U-15N], 1 – 3 mM; Tris-HCl, [U-98% 2H], 20 mM

Ex-cond_1: pH: 6.5; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1not availableEx-cond_1
2D 1H-1H TOCSYsample_1not availableEx-cond_1
2D 1H-15N HSQCsample_1not availableEx-cond_1
3D 1H-1H-15N NOESYsample_1not availableEx-cond_1
3D 1H-1H-15N TOCSYsample_1not availableEx-cond_1

Software:

NMRView - peak assignments

NMR spectrometers:

  • Varian UnityPlus 800 MHz

Related Database Links:

SWISS-PROT P28184
REF NP_038631
PRF 2102357B
PIR I67866
GenBank AAH59725 AAB31397 AAA39529
DBJ BAE24282
PDB

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