BMRB Entry 50651

Name: NMR assignment of T35S mutant of K-Ras4b(1-169) bound to GppNHp
Published: 2021-12-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50651

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of T35S mutant of K-Ras4b(1-169) bound to GppNHp

Deposition date:

2020-12-15

Original release date:

2021-12-14

Authors:

Sharma, Alok; Maciag, Anna

Citation:

Citation: Sharma, Alok; Dyba, Marcin; Tonelli, Marco; Smith, Brian; Gillette, William; Esposito, Dominic; Nissley, Dwight; McCormick, Frank; Maciag, Anna. "NMR 1 H, 13 C, 15 N backbone resonance assignments of the T35S and oncogenic T35S/Q61L mutants of human KRAS4b in the active, GppNHp-bound conformation" Biomol. NMR Assignments 16, 1-8 (2022).
PubMed: 34686998

Assembly members:

Assembly members:
entity_1, polymer, 169 residues, Formula weight is not available
entity_GNP, non-polymer, 522.196 Da.
entity_MG, non-polymer, 24.305 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTEYKLVVVGAGGVGKSALT IQLIQNHFVDEYDPSIEDSY RKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLC VFAINNTKSFEDIHHYREQI KRVKDSEDVPMVLVGNKSDL PSRTVDTKQAQDLARSYGIP FIETSAKTRQGVDDAFYTLV REIRKHKEK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	484
15N chemical shifts	164
1H chemical shifts	164

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GTPase K-Ras	1
2	GppNHp	2
3	Mg ion	3

Entities:

Entity 1, GTPase K-Ras 169 residues - Formula weight is not available

1

MET

THR

GLU

TYR

LYS

LEU

VAL

GLY

2

ALA

GLY

VAL

GLY

LYS

SER

ALA

LEU

THR

3

ILE

GLN

LEU

ILE

GLN

ASN

HIS

PHE

VAL

ASP

4

GLU

TYR

ASP

PRO

SER

ILE

GLU

ASP

SER

TYR

5

ARG

LYS

GLN

VAL

ILE

ASP

GLY

GLU

THR

6

CYS

LEU

ASP

ILE

LEU

ASP

THR

ALA

GLY

7

GLN

GLU

TYR

SER

ALA

MET

ARG

ASP

GLN

8

TYR

MET

ARG

THR

GLY

GLU

GLY

PHE

LEU

CYS

9

VAL

PHE

ALA

ILE

ASN

THR

LYS

SER

PHE

10

GLU

ASP

ILE

HIS

TYR

ARG

GLU

GLN

ILE

11

LYS

ARG

VAL

LYS

ASP

SER

GLU

ASP

VAL

PRO

12

MET

VAL

LEU

VAL

GLY

ASN

LYS

SER

ASP

LEU

13

PRO

SER

ARG

THR

VAL

ASP

THR

LYS

GLN

ALA

14

GLN

ASP

LEU

ALA

ARG

SER

TYR

GLY

ILE

PRO

15

PHE

ILE

GLU

THR

SER

ALA

LYS

THR

ARG

GLN

16

GLY

VAL

ASP

ALA

PHE

TYR

THR

LEU

VAL

17

ARG

GLU

ILE

ARG

LYS

HIS

LYS

GLU

LYS

Entity 2, GppNHp - C10 H17 N6 O13 P3 - 522.196 Da.

1

GNP

Entity 3, Mg ion - Mg - 24.305 Da.

1

MG

Samples:

sample_1: K-Ras4b(1-169), [U-99% 13C; U-99% 15N], 0.8 mM; K-Ras protein, [U-98% 15N], 0.8 mM; GppNHp 0.8 mM; MgCl2 2 mM; potassium chloride 100 mM; sodium chloride 50 mM; TCEP, [U-2H], 1 mM; MES, [U-2H], 20 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw v10.9 - Data were visualized and analyzed

NMRPipe v10.9 - Data were processed

CcpNMR v2.4 - NMR data analysis and assignments

NMR spectrometers:

Bruker Avance 700 MHz