BMRB Entry 50650

Name: human Angiogenin pH 6.3 35oC
Published: 2021-03-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50650

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

human Angiogenin pH 6.3 35oC

Deposition date:

2020-12-15

Original release date:

2021-03-15

Authors:

Fagagnini, Andrea; Gotte, Giovanni; Laurents, Douglas

Citation:

Citation: Fagagnini, Andrea; Garavis, Miguel; Gomez-Pinto, Irene; Fasoli, Sabrina; Gotte, Giovanni; Laurents, Douglas. "NMR Characterization of Angiogenin Variants and tRNA Ala Products Impacting Aberrant Protein Oligomerization" Int. J. Mol. Sci. 22, 1439-1439 (2021).
PubMed: 33535464

Assembly members:

Assembly members:
entity_1, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pAF

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QDNSRYTHFLTQHYDAKPQG RDDRYCESIMRRRGLTSPCK DINTFIHGNKRSIKAICENK NGNPHRENLRISKSSFQVTT CKLHGGSPWPPCQYRATAGF RNVVVACENGLPVHLDQSIF RRP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	283
15N chemical shifts	100
1H chemical shifts	187

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human Angiogenin monomer	1

Entities:

Entity 1, human Angiogenin monomer 123 residues - Formula weight is not available

1

GLN

ASP

ASN

SER

ARG

TYR

THR

HIS

PHE

LEU

2

THR

GLN

HIS

TYR

ASP

ALA

LYS

PRO

GLN

GLY

3

ARG

ASP

ARG

TYR

CYS

GLU

SER

ILE

MET

4

ARG

GLY

LEU

THR

SER

PRO

CYS

LYS

5

ASP

ILE

ASN

THR

PHE

ILE

HIS

GLY

ASN

LYS

6

ARG

SER

ILE

LYS

ALA

ILE

CYS

GLU

ASN

LYS

7

ASN

GLY

ASN

PRO

HIS

ARG

GLU

ASN

LEU

ARG

8

ILE

SER

LYS

SER

PHE

GLN

VAL

THR

9

CYS

LYS

LEU

HIS

GLY

SER

PRO

TRP

PRO

10

PRO

CYS

GLN

TYR

ARG

ALA

THR

ALA

GLY

PHE

11

ARG

ASN

VAL

ALA

CYS

GLU

ASN

GLY

12

LEU

PRO

VAL

HIS

LEU

ASP

GLN

SER

ILE

PHE

13

ARG

PRO

Samples:

sample_1: human Angiogenin, [U-98% 13C; U-98% 15N], 0.75 ± 0.2 mM; Na2HPO4 / NaH2PO4 buffer 50 mM

sample_conditions_1: ionic strength: 0.10 M; pH: 6.5; pressure: 0.92 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 15N	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1 - collection, processing

SPARKY v3.13 - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks