BMRB Entry 50642

Name: 1H, 13C, 15N Chemical Shift Assignments for the L75G mutant of Myristoylated HIV-1 Matrix
Published: 2021-01-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50642

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N Chemical Shift Assignments for the L75G mutant of Myristoylated HIV-1 Matrix

Deposition date:

2020-12-14

Original release date:

2021-01-26

Authors:

Eastep, Gunnar; Ghanam, Ruba; Green, Todd; Saad, Jamil

Citation:

Citation: Eastep, Gunnar; Ghanam, Ruba; Green, Todd; Saad, Jamil. "Structural characterization of HIV-1 matrix mutants implicated in envelope incorporation" J. Biol. Chem. 296, 100321-100321 (2021).
PubMed: 33485964

Assembly members:

Assembly members:
entity_1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-19b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XGARASVLSGGELDKWEKIR LRPGGKKQYKLKHIVWASRE LERFAVNPGLLETSEGCRQI LGQLQPSLQTGSEEGRSLYN TIAVLYCVHQRIDVKDTKEA LDKIEEEQNKSKKKAQQAAA DTGNNSQVSQNYHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	127
15N chemical shifts	122
1H chemical shifts	122

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HIV-1_L75G_myrMA	1

Entities:

Entity 1, HIV-1_L75G_myrMA 138 residues - Formula weight is not available

1

MYR

GLY

ALA

ARG

ALA

SER

VAL

LEU

SER

GLY

2

GLY

GLU

LEU

ASP

LYS

TRP

GLU

LYS

ILE

ARG

3

LEU

ARG

PRO

GLY

LYS

GLN

TYR

LYS

4

LEU

LYS

HIS

ILE

VAL

TRP

ALA

SER

ARG

GLU

5

LEU

GLU

ARG

PHE

ALA

VAL

ASN

PRO

GLY

LEU

6

LEU

GLU

THR

SER

GLU

GLY

CYS

ARG

GLN

ILE

7

LEU

GLY

GLN

LEU

GLN

PRO

SER

LEU

GLN

THR

8

GLY

SER

GLU

GLY

ARG

SER

LEU

TYR

ASN

9

THR

ILE

ALA

VAL

LEU

TYR

CYS

VAL

HIS

GLN

10

ARG

ILE

ASP

VAL

LYS

ASP

THR

LYS

GLU

ALA

11

LEU

ASP

LYS

ILE

GLU

GLN

ASN

LYS

12

SER

LYS

ALA

GLN

ALA

13

ASP

THR

GLY

ASN

SER

GLN

VAL

SER

GLN

14

ASN

TYR

HIS

Samples:

sample_1: HIV-1 L75G myrMA, [U-100% 13C; U-100% 15N], 0.250 mM; sodium phosphate 50 mM; sodium chloride 50 mM; TCEP 1 mM

sample_conditions_1: pH: 6.0; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMRPipe - processing

istHMS - processing

NMRDraw - processing

NMR spectrometers:

Bruker AVANCE II 700 MHz
Bruker AVANCE III-HD 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks