BMRB Entry 50630
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50630
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Citation: Araujo, Nelson; Bruix, Marta; Laurents, Douglas. "Disorder and partial folding in the regulatory subunit hinge region of Trypanosoma brucei protein kinase A: The C-linker portion inhibits the parasite's protein kinase A" Arch. Biochem. Biophys. 698, 108731-108731 (2021).
PubMed: 33359563
Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available
Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei
Experimental source: Production method: Synthetic peptides
Entity Sequences (FASTA):
entity_1: GGRKRRTTVRGEGIDPEKAK
SYVAPY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 48 |
| 1H chemical shifts | 181 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Hinge region | 1 |
Entities:
Entity 1, Hinge region 26 residues - Formula weight is not available
| 1 | GLY | GLY | ARG | LYS | ARG | ARG | THR | THR | VAL | ARG | ||||
| 2 | GLY | GLU | GLY | ILE | ASP | PRO | GLU | LYS | ALA | LYS | ||||
| 3 | SER | TYR | VAL | ALA | PRO | TYR |
Samples:
sample_1: Hinge region of the PKA regulatory subunit 1.9 mM; DSS 0.04 mM
sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2.1 - Data acquisition
SPARKY v3.114 - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz