BMRB Entry 50622

Name: nsp9 viral protein
Published: 2021-05-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50622

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

nsp9 viral protein

Deposition date:

2020-12-08

Original release date:

2021-05-28

Authors:

Dudas, Erika; Puglisi, Rita; Pastore, Annalisa

Citation:

Citation: Dudas, Erika; Puglisi, Rita; Korn, Sophie Marianne; Alfano, Caterina; Bellone, Maria Laura; Piaz, Fabrizio Dal; Kelly, Geoff; Monaca, Elisa; Schlundt, Andreas; Schwalbe, Harald; Pastore, Annalisa. "Backbone chemical shift spectral assignments of SARS coronavirus-2 non-structural protein nsp9." Biomol. NMR Assign. 15, 235-241 (2021).
PubMed: 33755914

Assembly members:

Assembly members:
entity_1, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKM263

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGNNELSPVALRQMSCAA GTTQTACTDDNALAYYNTTK GGRFVLALLSDLQDLKWARF PKSDGTGTIYTELEPPCRFV TDTPKGPKVKYLYFIKGLNN LNRGMVLGSLAATVRLQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	219
15N chemical shifts	80
1H chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	nsp9, chain 1	1
2	nsp9, chain 2	1

Entities:

Entity 1, nsp9, chain 1 117 residues - Formula weight is not available

GAMGNNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQ

1

GLY

ALA

MET

GLY

ASN

GLU

LEU

SER

PRO

2

VAL

ALA

LEU

ARG

GLN

MET

SER

CYS

ALA

3

GLY

THR

GLN

THR

ALA

CYS

THR

ASP

4

ASN

ALA

LEU

ALA

TYR

ASN

THR

LYS

5

GLY

ARG

PHE

VAL

LEU

ALA

LEU

SER

6

ASP

LEU

GLN

ASP

LEU

LYS

TRP

ALA

ARG

PHE

7

PRO

LYS

SER

ASP

GLY

THR

GLY

THR

ILE

TYR

8

THR

GLU

LEU

GLU

PRO

CYS

ARG

PHE

VAL

9

THR

ASP

THR

PRO

LYS

GLY

PRO

LYS

VAL

LYS

10

TYR

LEU

TYR

PHE

ILE

LYS

GLY

LEU

ASN

11

LEU

ASN

ARG

GLY

MET

VAL

LEU

GLY

SER

LEU

12

ALA

THR

VAL

ARG

LEU

GLN

Samples:

sample_1: SARS-CoV-2 nsp9 A, [U-99% 13C; U-99% 15N], 180 uM; sodium phosphate buffer 25 mM; NaCl 150 mM; TCEP 2 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
HNCACB	sample_1	isotropic	sample_conditions_1
NOESY-HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR, Topspin (Bruker), NMRPipe, SMILE - chemical shift assignment

NMR spectrometers:

Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks