BMRB Entry 50619

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50619

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Title:
1H 13C 15N assignment of IDR1-domain (1-47) of Nucleocapsid Protein of SARS-CoV 2 in the 1-248 construct
Deposition date:
2020-12-04
Original release date:
2021-03-11
Authors:
Schiavina, Marco; Pontoriero, Letizia; Felli, Isabella Caterina; Pierattelli, Roberta
Citation:

Citation: Schiavina, Marco; Pontoriero, Letizia; Uversky, Vladimir; Felli, Isabella Caterina; Pierattelli, Roberta. "The highly flexible, disordered regions of the SARS-CoV 2 Nucleocapsid protein within the 1-248 residue construct: sequence specific resonance assignments through NMR"  Biomol. NMR Assignments 15, 219-227 (2021).
PubMed: 33660218

Assembly members:

Assembly members:
entity_1, polymer, 248 residues, 26500 Da.

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDNGPQNQRNAPRITFGGP SDSTGSNQNGERSGARSKQR RPQGLPNNTASWFTALTQHG KEDLKFPRGQGVPINTNSSP DDQIGYYRRATRRIRGGDGK MKDLSPRWYFYYLGTGPEAG LPYGANKDGIIWVATEGALN TPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGS QASSRSSSRSRNSSRNSTPG SSRGTSPARMAGNGGDAALA LLLLDRLNQLESKMSGKGQQ QQGQTVTK

Data sets:
Data typeCount
13C chemical shifts130
15N chemical shifts45
1H chemical shifts40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-arm1

Entities:

Entity 1, N-arm 248 residues - 26500 Da.

1   METSERASPASNGLYPROGLNASNGLNARG
2   ASNALAPROARGILETHRPHEGLYGLYPRO
3   SERASPSERTHRGLYSERASNGLNASNGLY
4   GLUARGSERGLYALAARGSERLYSGLNARG
5   ARGPROGLNGLYLEUPROASNASNTHRALA
6   SERTRPPHETHRALALEUTHRGLNHISGLY
7   LYSGLUASPLEULYSPHEPROARGGLYGLN
8   GLYVALPROILEASNTHRASNSERSERPRO
9   ASPASPGLNILEGLYTYRTYRARGARGALA
10   THRARGARGILEARGGLYGLYASPGLYLYS
11   METLYSASPLEUSERPROARGTRPTYRPHE
12   TYRTYRLEUGLYTHRGLYPROGLUALAGLY
13   LEUPROTYRGLYALAASNLYSASPGLYILE
14   ILETRPVALALATHRGLUGLYALALEUASN
15   THRPROLYSASPHISILEGLYTHRARGASN
16   PROALAASNASNALAALAILEVALLEUGLN
17   LEUPROGLNGLYTHRTHRLEUPROLYSGLY
18   PHETYRALAGLUGLYSERARGGLYGLYSER
19   GLNALASERSERARGSERSERSERARGSER
20   ARGASNSERSERARGASNSERTHRPROGLY
21   SERSERARGGLYTHRSERPROALAARGMET
22   ALAGLYASNGLYGLYASPALAALALEUALA
23   LEULEULEULEUASPARGLEUASNGLNLEU
24   GLUSERLYSMETSERGLYLYSGLYGLNGLN
25   GLNGLNGLYGLNTHRVALTHRLYS

Samples:

sample_1: N -domain (1-47) of Nucleocapsid phosphoprotein of SARS-CoV2 in the 1-248 construct, [U-100% 13C; U-100% 15N], 300 uM; sodium chloride 450 mM; TRIS 25 mM; sodium azide 0.02 % v/v; D2O, [U-100% 2H], 5 % v/v

sample_2: N -domain (1-47) of Nucleocapsid phosphoprotein of SARS-CoV2 in the 1-248 construct, [U-100% 13C; U-100% 15N], 300 uM; sodium chloride 450 mM; TRIS 25 mM; sodium azide 0.02 % v/v; D2O, [U-100% 2H], 5 % v/v

sample_conditions_1: ionic strength: 0.5 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N BEST TROSYsample_1isotropicsample_conditions_1
2D 13C-15N CONsample_2isotropicsample_conditions_1
3D 1H BT-HN(CO)CACBsample_1isotropicsample_conditions_1
3D 1H BT-HNCACBsample_1isotropicsample_conditions_1
3D 1H HNCOsample_1isotropicsample_conditions_1
3D 1H HN(CA)COsample_1isotropicsample_conditions_1
3D 13C (H)CBCACONsample_2isotropicsample_conditions_1
3D 13C (H)CBCANCOsample_2isotropicsample_conditions_1
2D (H)CACOsample_2isotropicsample_conditions_1
2D (H)CBCACOsample_2isotropicsample_conditions_1

Software:

XEASY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz
  • Bruker AVANCE NEO 1200 MHz

Related Database Links:

UNP P0DTC9
AlphaFold P0DTC9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks