BMRB Entry 50604

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50604

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Title:
Human Histone H2A type-2A in Nucleosome
Deposition date:
2020-11-30
Original release date:
2021-02-13
Authors:
Witus, Samuel; Tuttle, Lisa; Klevit, Rachel
Citation:

Citation: Witus, Samuel; Burrell, Anika; Farrell, Daniel; Kang, Jianming; Wang, Meiling; Hansen, Jesse; Pravat, Alex; Tuttle, Lisa; Stewart, Mikaela; Brzovic, Peter; Chatterjee, Champak; Zhao, Weixing; DiMaio, Frank; Kollman, Justin; Klevit, Rachel. "BRCA1/BARD1 site-specific ubiquitylation of nucleosomal H2A is directed by BARD1"  Nat. Struct. Mol. Biol. 28, 268-277 (2021).
PubMed: 33589814

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available
entity_2, polymer, 125 residues, Formula weight is not available
entity_3, polymer, 135 residues, Formula weight is not available
entity_4, polymer, 102 residues, Formula weight is not available
entity_5, polymer, 153 residues, Formula weight is not available
entity_6, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTEV19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGSGRGKQGGKARAKAKS RSSRAGLQFPVGRVHRLLRK GNYAERVGAGAPVYMAAVLE YLTAEILELAGNAARDNKKT RIIPRHLQLAIRNDEELNKL LGKVTIAQGGVLPNIQAVLL PKKTESHHKAKGK
entity_2: PEPAKSAPAPKKGSKKAVTK AQKKDGKKRKRSRKESYSVY VYKVLKQVHPDTGISSKAMG IMNSFVNDIFERIAGEASRL AHYNKRSTITSREIQTAVRL LLPGELAKHAVSEGTKAVTK YTSAK
entity_3: ARTKQTARKSTGGKAPRKQL ATKAARKSAPATGGVKKPHR YRPGTVALREIRRYQKSTEL LIRKLPFQRLVREIAQDFKT DLRFQSSAVMALQEASEAYL VGLFEDTNLAAIHAKRVTIM PKDIQLARRIRGERA
entity_4: SGRGKGGKGLGKGGAKRHRK VLRDNIQGITKPAIRRLARR GGVKRISGLIYEETRGVLKV FLENVIRDAVTYTEHAKRKT VTAMDVVYALKRQGRTLYGF GG
entity_5: ATCCTGGAGAATCCCGGTGC CGAGGCCGCTCAATTGGTCG TAGACAGCTCTAGCACCGCT TAAACGCACGTACGCGCTGT CCCCCGCGTTTTAACCGCCA AGGGGATTACTCCCTAGTCT CCAGGCACGTGTCAGATATA TACATCCTGTGAT
entity_6: ATCACAGGATGTATATATCT GACACGTGCCTGGAGACTAG GGAGTAATCCCCTTGGCGGT TAAAACGCGGGGGACAGCGC GTACGTGCGTTTAAGCGGTG CTAGAGCTGTCTACGACCAA TTGAGCGGCCTCGGCACCGG GATTCTCCAGGAT

Data sets:
Data typeCount
13C chemical shifts33
15N chemical shifts19
1H chemical shifts20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H2A1
2H2B2
3H33
4H44
5Widom 601_15
6Widom 602_26

Entities:

Entity 1, H2A 133 residues - Formula weight is not available

Human histone H2A type-2A. Two copies per nucleosome. This is the 2H, 15N, 13C labelled component in sample.

1   GLYALAMETGLYSERGLYARGGLYLYSGLN
2   GLYGLYLYSALAARGALALYSALALYSSER
3   ARGSERSERARGALAGLYLEUGLNPHEPRO
4   VALGLYARGVALHISARGLEULEUARGLYS
5   GLYASNTYRALAGLUARGVALGLYALAGLY
6   ALAPROVALTYRMETALAALAVALLEUGLU
7   TYRLEUTHRALAGLUILELEUGLULEUALA
8   GLYASNALAALAARGASPASNLYSLYSTHR
9   ARGILEILEPROARGHISLEUGLNLEUALA
10   ILEARGASNASPGLUGLULEUASNLYSLEU
11   LEUGLYLYSVALTHRILEALAGLNGLYGLY
12   VALLEUPROASNILEGLNALAVALLEULEU
13   PROLYSLYSTHRGLUSERHISHISLYSALA
14   LYSGLYLYS

Entity 2, H2B 125 residues - Formula weight is not available

Human histone H2B type 1K. Two copies per nucleosome.

1   PROGLUPROALALYSSERALAPROALAPRO
2   LYSLYSGLYSERLYSLYSALAVALTHRLYS
3   ALAGLNLYSLYSASPGLYLYSLYSARGLYS
4   ARGSERARGLYSGLUSERTYRSERVALTYR
5   VALTYRLYSVALLEULYSGLNVALHISPRO
6   ASPTHRGLYILESERSERLYSALAMETGLY
7   ILEMETASNSERPHEVALASNASPILEPHE
8   GLUARGILEALAGLYGLUALASERARGLEU
9   ALAHISTYRASNLYSARGSERTHRILETHR
10   SERARGGLUILEGLNTHRALAVALARGLEU
11   LEULEUPROGLYGLULEUALALYSHISALA
12   VALSERGLUGLYTHRLYSALAVALTHRLYS
13   TYRTHRSERALALYS

Entity 3, H3 135 residues - Formula weight is not available

Human histone H3.2. Two copies per nucleosome.

1   ALAARGTHRLYSGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU
3   ALATHRLYSALAALAARGLYSSERALAPRO
4   ALATHRGLYGLYVALLYSLYSPROHISARG
5   TYRARGPROGLYTHRVALALALEUARGGLU
6   ILEARGARGTYRGLNLYSSERTHRGLULEU
7   LEUILEARGLYSLEUPROPHEGLNARGLEU
8   VALARGGLUILEALAGLNASPPHELYSTHR
9   ASPLEUARGPHEGLNSERSERALAVALMET
10   ALALEUGLNGLUALASERGLUALATYRLEU
11   VALGLYLEUPHEGLUASPTHRASNLEUALA
12   ALAILEHISALALYSARGVALTHRILEMET
13   PROLYSASPILEGLNLEUALAARGARGILE
14   ARGGLYGLUARGALA

Entity 4, H4 102 residues - Formula weight is not available

Human histone H4. Two copies per nucleosome.

1   SERGLYARGGLYLYSGLYGLYLYSGLYLEU
2   GLYLYSGLYGLYALALYSARGHISARGLYS
3   VALLEUARGASPASNILEGLNGLYILETHR
4   LYSPROALAILEARGARGLEUALAARGARG
5   GLYGLYVALLYSARGILESERGLYLEUILE
6   TYRGLUGLUTHRARGGLYVALLEULYSVAL
7   PHELEUGLUASNVALILEARGASPALAVAL
8   THRTYRTHRGLUHISALALYSARGLYSTHR
9   VALTHRALAMETASPVALVALTYRALALEU
10   LYSARGGLNGLYARGTHRLEUTYRGLYPHE
11   GLYGLY

Entity 5, Widom 601_1 153 residues - Formula weight is not available

Widom 601 dsDNA strand 1. 153 bases.

1   DADTDCDCDTDGDGDADGDA
2   DADTDCDCDCDGDGDTDGDC
3   DCDGDADGDGDCDCDGDCDT
4   DCDADADTDTDGDGDTDCDG
5   DTDADGDADCDADGDCDTDC
6   DTDADGDCDADCDCDGDCDT
7   DTDADADADCDGDCDADCDG
8   DTDADCDGDCDGDCDTDGDT
9   DCDCDCDCDCDGDCDGDTDT
10   DTDTDADADCDCDGDCDCDA
11   DADGDGDGDGDADTDTDADC
12   DTDCDCDCDTDADGDTDCDT
13   DCDCDADGDGDCDADCDGDT
14   DGDTDCDADGDADTDADTDA
15   DTDADCDADTDCDCDTDGDT
16   DGDADT

Entity 6, Widom 602_2 153 residues - Formula weight is not available

Widom 601 dsDNA strand 2. 153 bases.

1   DADTDCDADCDADGDGDADT
2   DGDTDADTDADTDADTDCDT
3   DGDADCDADCDGDTDGDCDC
4   DTDGDGDADGDADCDTDADG
5   DGDGDADGDTDADADTDCDC
6   DCDCDTDTDGDGDCDGDGDT
7   DTDADADADADCDGDCDGDG
8   DGDGDGDADCDADGDCDGDC
9   DGDTDADCDGDTDGDCDGDT
10   DTDTDADADGDCDGDGDTDG
11   DCDTDADGDADGDCDTDGDT
12   DCDTDADCDGDADCDCDADA
13   DTDTDGDADGDCDGDGDCDC
14   DTDCDGDGDCDADCDCDGDG
15   DGDADTDTDCDTDCDCDADG
16   DGDADT

Samples:

sample_1: Nucleosome Core Particle (human) 200 uM; Histone H2A in nucleosome core particle, [U-100% 13C; U-100% 15N; U-80% 2H], 400 uM; MOPS 20 mM; sodium chloride 50 mM; TCEP 0.5 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1 - collection

NMRPipe - processing

NMRViewJ - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks