BMRB Entry 50602
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50602
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Citation: Fischer, Elyse; Yu, Conny; Bellini, Dom; McLaughlin, Stephen; Orr, Christian; Wagner, Armin; Freund, Stefan; Barford, David. "Molecular mechanism of Mad1 kinetochore targeting by phosphorylated Bub1" EMBO Reports 19, e52242-e52242 (2021).
PubMed: 34013668
Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSFDuet-1
Entity Sequences (FASTA):
entity_1: SSKEVAELKKQVESAELKNQ
RLKEVFQTKIQEFRKACYTL
TGYQIDITTENQYRLTSLYA
EHPGDCLIFKATSPSGSKMQ
LLETEFSHTVGELIEVHLRR
QDSIPAFLSSLTLELFSRQT
VA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 339 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 112 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mad1-CTD | 1 |
Entities:
Entity 1, Mad1-CTD 122 residues - Formula weight is not available
| 1 | SER | SER | LYS | GLU | VAL | ALA | GLU | LEU | LYS | LYS | ||||
| 2 | GLN | VAL | GLU | SER | ALA | GLU | LEU | LYS | ASN | GLN | ||||
| 3 | ARG | LEU | LYS | GLU | VAL | PHE | GLN | THR | LYS | ILE | ||||
| 4 | GLN | GLU | PHE | ARG | LYS | ALA | CYS | TYR | THR | LEU | ||||
| 5 | THR | GLY | TYR | GLN | ILE | ASP | ILE | THR | THR | GLU | ||||
| 6 | ASN | GLN | TYR | ARG | LEU | THR | SER | LEU | TYR | ALA | ||||
| 7 | GLU | HIS | PRO | GLY | ASP | CYS | LEU | ILE | PHE | LYS | ||||
| 8 | ALA | THR | SER | PRO | SER | GLY | SER | LYS | MET | GLN | ||||
| 9 | LEU | LEU | GLU | THR | GLU | PHE | SER | HIS | THR | VAL | ||||
| 10 | GLY | GLU | LEU | ILE | GLU | VAL | HIS | LEU | ARG | ARG | ||||
| 11 | GLN | ASP | SER | ILE | PRO | ALA | PHE | LEU | SER | SER | ||||
| 12 | LEU | THR | LEU | GLU | LEU | PHE | SER | ARG | GLN | THR | ||||
| 13 | VAL | ALA |
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