BMRB Entry 5060

Title:
1H, 15N, 13C NMR Assignments of TM006 Protein from Thermotoga maritima
Deposition date:
2001-06-15
Original release date:
2002-02-20
Authors:
Denisov, Alexei; Finak, Grzegorz; Gehring, Kalle
Citation:

Citation: Yee, Adelinda; Chang, Xiaoqing; Pineda-Lucena, Antonio; Wu, Bin; Semesi, Anthony; Le, Brian; Ramelot, Theresa; Lee, Gregory; Bhattacharyya, Sudeepa; Gutierrez, Pablo; Denisov, Alexei; Lee, Chang-Hun; Cort, John; Kozlov, Guennadi; Liao, Jack; Finak, Grzegorz; Chen, Limin; Wishart, David; Lee, Weontae; McIntosh, Lawrence; Gehring, Kalle; Kennedy, Michael; Edwards, Aled; Arrowsmith, Cheryl. "An NMR approach to structural proteomics"  Proc. Natl. Acad. Sci. U.S.A. 99, 1825-1830 (2002).

Assembly members:

Assembly members:
TM006 protein from Thermotoga maritima, polymer, 98 residues, 11200 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts457
13C chemical shifts253
15N chemical shifts81
coupling constants79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TM0061

Entities:

Entity 1, TM006 98 residues - 11200 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   ALALYSTYRGLNVALTHRLYSTHRLEUASP
4   VALARGGLYGLUVALCYSPROVALPROASP
5   VALGLUTHRLYSARGALALEUGLNASNMET
6   LYSPROGLYGLUILELEUGLUVALTRPILE
7   ASPTYRPROMETSERLYSGLUARGILEPRO
8   GLUTHRVALLYSLYSLEUGLYHISGLUVAL
9   LEUGLUILEGLUGLUVALGLYPROSERGLU
10   TRPLYSILETYRILELYSVALLYS

Samples:

sample_1: TM006 protein from Thermotoga maritima, [U-99% 13C; U-99% 15N], 2 mM; H20 90%; D20 10%

sample_2: TM006 protein from Thermotoga maritima, [U-99% 15N], 2 mM; H20 90%; D20 10%

sample_3: TM006 protein from Thermotoga maritima 5 mM; H20 90%; D20 10%

Ex-cond_1: pH: 6.8; temperature: 310 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)HNnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D 15N-HMQC-NOESYnot availablenot availablenot available
3D 15N-HMQC-TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D 15N-HSQCnot availablenot availablenot available
2D 13C-HSQCnot availablenot availablenot available

Software:

XWINNMR v2.1 - data collection, data processing

XEASY v1.3.13 - data analysis

ARIA v14.5.98 - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAD36062 ABQ47759 ACB10143 ACM23771 ADA67833
REF NP_228791 WP_004080581 WP_013932246 WP_015920041 YP_001245335
SP Q9X078
AlphaFold Q9X078

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks