BMRB Entry 50593

Title:
nsP3 VEEV
Deposition date:
2020-11-23
Original release date:
2021-06-30
Authors:
Agback, Peter; Shernyukov, Andrey; Dominguez, Francisco; Agback, Tatiana; Frolova, Elena
Citation:

Citation: Agback, Peter; Shernyukov, Andrey; Dominguez, Francisco; Agback, Tatiana; Frolova, Elena. "Novel NMR Assignment Strategy Reveals Structural Heterogeneity in Solution of the nsP3 HVD Domain of Venezuelan Equine Encephalitis Virus."  Molecules 25, 5824-5824 (2020).
PubMed: 33321815

Assembly members:

Assembly members:
entity_1, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: alphavirus togaviridae   Taxonomy ID: 11018   Superkingdom: Viruses   Kingdom: not available   Genus/species: alphavirus togaviridae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pE-SUMOpro-3

Data sets:
Data typeCount
13C chemical shifts517
15N chemical shifts150
1H chemical shifts332

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nsP31

Entities:

Entity 1, nsP3 189 residues - Formula weight is not available

1   GLYSERLEUTRPVALGLUTHRPROPROVAL
2   ASPGLUTHRPROGLUPROSERALAGLUASN
3   GLNSERTHRGLUGLYTHRPROGLUGLNPRO
4   PROLEUILETHRGLUASPGLUTHRARGTHR
5   ARGTHRPROGLUPROILEILEILEGLUGLU
6   GLUGLUGLUASPSERILESERLEULEUSER
7   ASPGLYPROTHRHISGLNVALLEUGLNVAL
8   GLUALAASPILEHISGLYPROPROSERVAL
9   SERSERSERSERTRPSERILEPROHISALA
10   SERASPPHEASPVALASPSERLEUSERILE
11   LEUASPTHRLEUGLUGLYALASERVALTHR
12   SERGLYALATHRSERALAGLUTHRASNSER
13   TYRPHEALALYSSERMETGLUPHELEUALA
14   ARGPROVALPROALAPROARGTHRVALPHE
15   ARGASNPROPROHISPROALAPROARGTHR
16   ARGTHRPROSERLEUALAPROSERARGALA
17   CYSSERARGTHRSERLEUVALSERTHRPRO
18   PROGLYVALASNARGVALILETHRARGGLU
19   GLULEUGLUALALEUTHRPROSERARG

Samples:

sample_1: nsP3, [U-100% 13C; U-100% 15N], 765 ± 15 uM; TCEP 2 mM; NaN3 1 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - collection

TOPSPIN v4.0.5 - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks