BMRB Entry 50590

Name: Backbone assignments of WRKY1-C(V273-P343) from Arabidopsis thaliana
Published: 2024-10-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50590

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of WRKY1-C(V273-P343) from Arabidopsis thaliana

Deposition date:

2020-11-22

Original release date:

2024-10-23

Authors:

Yu, Lulu; Li, Qianwen; Hu, Yunfei

Citation:

Citation: Yu, Lulu; Li, Qianwen; Hu, Yunfei. "Structural basis for the regulation of WRKY transcription factors by the intrinsically disordered VQ proteins" Nucleic Acids Res. Suppl. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MVHTQTLFDIVNDGYRWRKY GQKSVKGSPYPRSYYRCSSP GCPVKKHVERSSHDTKLLIT TYEGKHDHDMPP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	47
1H chemical shifts	47

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WRKY1-C(273-343)	1

Entities:

Entity 1, WRKY1-C(273-343) 72 residues - Formula weight is not available

1

MET

VAL

HIS

THR

GLN

THR

LEU

PHE

ASP

ILE

2

VAL

ASN

ASP

GLY

TYR

ARG

TRP

ARG

LYS

TYR

3

GLY

GLN

LYS

SER

VAL

LYS

GLY

SER

PRO

TYR

4

PRO

ARG

SER

TYR

ARG

CYS

SER

PRO

5

GLY

CYS

PRO

VAL

LYS

HIS

VAL

GLU

ARG

6

SER

HIS

ASP

THR

LYS

LEU

ILE

THR

7

THR

TYR

GLU

GLY

LYS

HIS

ASP

HIS

ASP

MET

8

PRO

Samples:

sample_1: WRKY1-C(273-343), [U-95% 13C; U-95% 15N], 0.5 mM; D2O, [U-99% 2H], 10 % v/v; sodium chloride 100 mM; HEPES 25 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRView - chemical shift assignment, data analysis, processing

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks