BMRB Entry 50588

Name: 1H, 13C, and 15N resonance assignments of Arabidopsis WRKY1-N domain complexed with DNA
Published: 2024-10-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50588

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments of Arabidopsis WRKY1-N domain complexed with DNA

Deposition date:

2020-11-21

Original release date:

2024-10-23

Authors:

Yu, Lulu; Li, Qianwen; Hu, Yunfei

Citation:

Citation: Yu, Lulu; Li, Qianwen; Hu, Yunfei. "Structural basis for the regulation of WRKY transcription factors by the intrinsically disordered VQ proteins" Nucleic Acids Res. Suppl. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSLQSGSEGNSPFIREKVME DGYNWRKYGQKLVKGNEFVR SYYRCTHPNCKAKKQLERSA GGQVVDTVYFGEHDHPKPL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	266
15N chemical shifts	66
1H chemical shifts	398

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WRKY1-N	1

Entities:

Entity 1, WRKY1-N 79 residues - Formula weight is not available

1

MET

SER

LEU

GLN

SER

GLY

SER

GLU

GLY

ASN

2

SER

PRO

PHE

ILE

ARG

GLU

LYS

VAL

MET

GLU

3

ASP

GLY

TYR

ASN

TRP

ARG

LYS

TYR

GLY

GLN

4

LYS

LEU

VAL

LYS

GLY

ASN

GLU

PHE

VAL

ARG

5

SER

TYR

ARG

CYS

THR

HIS

PRO

ASN

CYS

6

LYS

ALA

LYS

GLN

LEU

GLU

ARG

SER

ALA

7

GLY

GLN

VAL

ASP

THR

VAL

TYR

PHE

8

GLY

GLU

HIS

ASP

HIS

PRO

LYS

PRO

LEU

Samples:

sample_1: WRKY1-N, [U-95% 13C; U-95% 15N], 0.5 mM; D2O, [U-99% 2H], 10 % v/v; sodium chloride 100 mM; HEPES 25 mM; DNA 0.5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRView - chemical shift assignment, data analysis, processing

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks