BMRB Entry 50586

Name: 1H, 13C, and 15N resonance assignments of WRKY1-C(265-343) from Arabidopsis thaliana
Published: 2024-10-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50586

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments of WRKY1-C(265-343) from Arabidopsis thaliana

Deposition date:

2020-11-20

Original release date:

2024-10-23

Authors:

Yu, Lulu; Li, Qianwen; Hu, Yunfei

Citation:

Citation: Yu, Lulu; Li, Qianwen; Hu, Yunfei. "Structural basis for the regulation of WRKY transcription factors by the intrinsically disordered VQ proteins" Nucleic Acids Res. Suppl. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSTNDSRIVVHTQTLFDIVN DGYRWRKYGQKSVKGSPYPR SYYRCSSPGCPVKKHVERSS HDTKLLITTYEGKHDHDMPP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	53
1H chemical shifts	53

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WRKY1-C(265-343)	1

Entities:

Entity 1, WRKY1-C(265-343) 80 residues - Formula weight is not available

1	MET	SER	THR	ASN	ASP	SER	ARG	ILE	VAL	VAL
2	HIS	THR	GLN	THR	LEU	PHE	ASP	ILE	VAL	ASN
3	ASP	GLY	TYR	ARG	TRP	ARG	LYS	TYR	GLY	GLN
4	LYS	SER	VAL	LYS	GLY	SER	PRO	TYR	PRO	ARG
5	SER	TYR	TYR	ARG	CYS	SER	SER	PRO	GLY	CYS
6	PRO	VAL	LYS	LYS	HIS	VAL	GLU	ARG	SER	SER
7	HIS	ASP	THR	LYS	LEU	LEU	ILE	THR	THR	TYR
8	GLU	GLY	LYS	HIS	ASP	HIS	ASP	MET	PRO	PRO

Samples:

sample_1: WRKY1-C(265-343), [U-95% 13C; U-90% 15N], 0.5 mM; D2O, [U-99% 2H], 10 % v/v; HEPES 25 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRView - chemical shift assignment, data analysis, processing

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	SER	THR	ASN	ASP	SER	ARG	ILE	VAL	VAL
2	HIS	THR	GLN	THR	LEU	PHE	ASP	ILE	VAL	ASN
3	ASP	GLY	TYR	ARG	TRP	ARG	LYS	TYR	GLY	GLN
4	LYS	SER	VAL	LYS	GLY	SER	PRO	TYR	PRO	ARG
5	SER	TYR	TYR	ARG	CYS	SER	SER	PRO	GLY	CYS
6	PRO	VAL	LYS	LYS	HIS	VAL	GLU	ARG	SER	SER
7	HIS	ASP	THR	LYS	LEU	LEU	ILE	THR	THR	TYR
8	GLU	GLY	LYS	HIS	ASP	HIS	ASP	MET	PRO	PRO

1	MET	SER	THR	ASN	ASP	SER	ARG	ILE	VAL	VAL
2	HIS	THR	GLN	THR	LEU	PHE	ASP	ILE	VAL	ASN
3	ASP	GLY	TYR	ARG	TRP	ARG	LYS	TYR	GLY	GLN
4	LYS	SER	VAL	LYS	GLY	SER	PRO	TYR	PRO	ARG
5	SER	TYR	TYR	ARG	CYS	SER	SER	PRO	GLY	CYS
6	PRO	VAL	LYS	LYS	HIS	VAL	GLU	ARG	SER	SER
7	HIS	ASP	THR	LYS	LEU	LEU	ILE	THR	THR	TYR
8	GLU	GLY	LYS	HIS	ASP	HIS	ASP	MET	PRO	PRO

1	MET	SER	THR	ASN	ASP	SER	ARG	ILE	VAL	VAL
2	HIS	THR	GLN	THR	LEU	PHE	ASP	ILE	VAL	ASN
3	ASP	GLY	TYR	ARG	TRP	ARG	LYS	TYR	GLY	GLN
4	LYS	SER	VAL	LYS	GLY	SER	PRO	TYR	PRO	ARG
5	SER	TYR	TYR	ARG	CYS	SER	SER	PRO	GLY	CYS
6	PRO	VAL	LYS	LYS	HIS	VAL	GLU	ARG	SER	SER
7	HIS	ASP	THR	LYS	LEU	LEU	ILE	THR	THR	TYR
8	GLU	GLY	LYS	HIS	ASP	HIS	ASP	MET	PRO	PRO