BMRB Entry 50583

Name: 1H, 13C, and 15N resonance assignments of combined SIB1
Published: 2024-09-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50583

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments of combined SIB1

Deposition date:

2020-11-19

Original release date:

2024-09-16

Authors:

Yu, Lulu; Hu, Yunfei

Citation:

Citation: Dong, Xu; Yu, Lulu; Zhang, Qiang; Yang, Ju; Gong, Zhou; Niu, Xiaogang; Li, Hongwei; Zhang, Xu; Liu, Maili; Jin, Changwen; Hu, Yunfei. "Structural basis for the regulation of plant transcription factor WRKY33 by the VQ protein SIB1" Commun. Biol. 7, 561-561 (2024).
PubMed: 38734744

Assembly members:

Assembly members:
entity_1, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTTSLDKKKPSPVSRKSPKQ KKKTTSTNKPIKVRYISNPM RVQTCASKFRELVQELTGQD AVDLQPEPIYSPSSDDHNLS PPAENLAPRVL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	116
15N chemical shifts	50
1H chemical shifts	51

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SIB1	1

Entities:

Entity 1, SIB1 91 residues - Formula weight is not available

1

MET

THR

SER

LEU

ASP

LYS

PRO

2

SER

PRO

VAL

SER

ARG

LYS

SER

PRO

LYS

GLN

3

LYS

THR

SER

THR

ASN

LYS

PRO

4

ILE

LYS

VAL

ARG

TYR

ILE

SER

ASN

PRO

MET

5

ARG

VAL

GLN

THR

CYS

ALA

SER

LYS

PHE

ARG

6

GLU

LEU

VAL

GLN

GLU

LEU

THR

GLY

GLN

ASP

7

ALA

VAL

ASP

LEU

GLN

PRO

GLU

PRO

ILE

TYR

8

SER

PRO

SER

ASP

HIS

ASN

LEU

SER

9

PRO

ALA

GLU

ASN

LEU

ALA

PRO

ARG

VAL

10

LEU

Samples:

sample_1: Combined SIB1, [U-95% 13C; U-95% 15N], 0.5 mM; WRKY33 0.5 mM; D2O, [U-99% 2H], 10 % v/v

sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRView - chemical shift assignment, data analysis, processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks