BMRB Entry 50580
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50580
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Dong, Xu; Yu, Lulu; Zhang, Qiang; Yang, Ju; Gong, Zhou; Niu, Xiaogang; Li, Hongwei; Zhang, Xu; Liu, Maili; Jin, Changwen; Hu, Yunfei. "Structural basis for the regulation of plant transcription factor WRKY33 by the VQ protein SIB1" Commun. Biol. 7, 561-561 (2024).
PubMed: 38734744
Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a
Entity Sequences (FASTA):
entity_1: MDNETNGGNGGGSKTVREPR
IVVQTTSDIDILDDGYRWRK
YGQKVVKGNPNPRSYYKCTT
IGCPVRKHVERASHDMRAVI
TTYEGKHNHDVPA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 340 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 484 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WRKY33-C(D331-A422) | 1 |
Entities:
Entity 1, WRKY33-C(D331-A422) 93 residues - Formula weight is not available
| 1 | MET | ASP | ASN | GLU | THR | ASN | GLY | GLY | ASN | GLY | ||||
| 2 | GLY | GLY | SER | LYS | THR | VAL | ARG | GLU | PRO | ARG | ||||
| 3 | ILE | VAL | VAL | GLN | THR | THR | SER | ASP | ILE | ASP | ||||
| 4 | ILE | LEU | ASP | ASP | GLY | TYR | ARG | TRP | ARG | LYS | ||||
| 5 | TYR | GLY | GLN | LYS | VAL | VAL | LYS | GLY | ASN | PRO | ||||
| 6 | ASN | PRO | ARG | SER | TYR | TYR | LYS | CYS | THR | THR | ||||
| 7 | ILE | GLY | CYS | PRO | VAL | ARG | LYS | HIS | VAL | GLU | ||||
| 8 | ARG | ALA | SER | HIS | ASP | MET | ARG | ALA | VAL | ILE | ||||
| 9 | THR | THR | TYR | GLU | GLY | LYS | HIS | ASN | HIS | ASP | ||||
| 10 | VAL | PRO | ALA |
Samples:
sample_1: WRKY33-C(d331-a422), [U-95% 13C; U-95% 15N], 0.5 mM; D2O, [U-99% 2H], 10 % v/v
sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRView - chemical shift assignment, data analysis, processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks