BMRB Entry 50579

Name: 1H, 13C, and 15N resonance assignments of WRKY33-C(V352-A422) from Arabidopsis thaliana
Published: 2024-09-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50579

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments of WRKY33-C(V352-A422) from Arabidopsis thaliana

Deposition date:

2020-11-19

Original release date:

2024-09-16

Authors:

Yu, Lulu; Hu, Yunfei

Citation:

Citation: Dong, Xu; Yu, Lulu; Zhang, Qiang; Yang, Ju; Gong, Zhou; Niu, Xiaogang; Li, Hongwei; Zhang, Xu; Liu, Maili; Jin, Changwen; Hu, Yunfei. "Structural basis for the regulation of plant transcription factor WRKY33 by the VQ protein SIB1" Commun. Biol. 7, 561-561 (2024).
PubMed: 38734744

Assembly members:

Assembly members:
entity_1, polymer, 72 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MVQTTSDIDILDDGYRWRKY GQKVVKGNPNPRSYYKCTTI GCPVRKHVERASHDMRAVIT TYEGKHNHDVPA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	273
15N chemical shifts	65
1H chemical shifts	428

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WRKY33-C(352-422)	1
2	ZINC ION	2

Entities:

Entity 1, WRKY33-C(352-422) 72 residues - Formula weight is not available

1

MET

VAL

GLN

THR

SER

ASP

ILE

ASP

ILE

2

LEU

ASP

GLY

TYR

ARG

TRP

ARG

LYS

TYR

3

GLY

GLN

LYS

VAL

LYS

GLY

ASN

PRO

ASN

4

PRO

ARG

SER

TYR

LYS

CYS

THR

ILE

5

GLY

CYS

PRO

VAL

ARG

LYS

HIS

VAL

GLU

ARG

6

ALA

SER

HIS

ASP

MET

ARG

ALA

VAL

ILE

THR

7

THR

TYR

GLU

GLY

LYS

HIS

ASN

HIS

ASP

VAL

8

PRO

ALA

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: WRKY33-C(352-422), [U-95% 13C; U-95% 15N], 0.5 mM; MES 30 mM; sodium chloride 50 mM; D2O, [U-99% 2H], 10 % v/v

sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRView - chemical shift assignment, data analysis, processing

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks