BMRB Entry 50572

Name: PfAlu RNA Helix5
Published: 2021-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50572

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

PfAlu RNA Helix5

Deposition date:

2020-11-12

Original release date:

2021-05-11

Authors:

Soni, Komal; Sinning, Irmgard

Citation:

Citation: Soni, Komal; Kempf, Georg; Manalastas-Cantos, Karen; Hendricks, Astrid; Flemming, Dirk; Guizetti, Julien; Simon, Bernd; Frischknecht, Friedrich; Svergun, Dmitri; Wild, Klemens; Sinning, Irmgard. "Structural analysis of the SRP Alu domain from Plasmodium falciparum reveals a non-canonical open conformation" Commun. Biol. 4, 600-600 (2021).
PubMed: 34017052

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Plasmodium falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCUAUAAUAUUUCUGAGAG UAAUCUCAUAAGUAUAAUAU GUA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	15

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PfAlu Helix5	1

Entities:

Entity 1, PfAlu Helix5 43 residues - Formula weight is not available

Due to insertion of a closing tetraloop inplace of the S-domain, the numbering is such that after the tetraloop at A98, orginal sequence numbering from U279 to U298 is continued.

1

G

C

U

A

U

A

U

A

2

U

C

U

G

A

G

A

G

3

U

A

U

C

U

C

A

U

A

4

A

G

U

A

U

A

U

A

U

5

G

U

A

Samples:

sample_1: PfAlu Helix5 275 uM; sodium phosphate 20 mM

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz